Atula S, Auvinen E, Grenman R, Syrjänen S
MediCity Research Center, University of Turku, Finland.
Anticancer Res. 1997 Nov-Dec;17(6D):4427-33.
Human papillomavirus (HPV) DNA has been detected in carcinomas of the upper aerodigestive tract. However, studies of the subject show considerable variation in their results, and the causal relationship between HPV and squamous cell carcinomas of the head and neck area still remains to be determined. Epstein-Barr virus (EBV) is consistently detected in nasopharyngeal carcinoma lesions, but little is known about its association with other carcinomas of the head and neck region. The present study was carried out on the role of HPV and EBV in epithelial carcinomas of the upper aerodigestive tract.
The study material comprised 79 frozen biopsy samples from epithelial head and neck carcinomas. DNA was extracted from frozen biopsy samples for analysis using Southern blot hybridization (SBH) and polymerase chain reaction (PCR) to detect HPV DNA.
HPV DNA was detected in 13 samples (16.5%) by SBH under low stringency conditions and in three samples (3.8%) by PCR using general primers targeting the HPV L1 region. HPV seemed to have affinity for labial carcinomas: four of the six samples (66.7%) were HPV DNA positive. The detection rate of HPV diminished from the labial and oral epithelium towards the laryngeal region. In SBH, EBV DNA was not found in any of the biopsy samples.
HPV seems to be involved in multifactorial carcinogenesis in the head and neck epithelium, but the association is not as evident as that found in genital carcinomas. The results suggest that EBV is not associated with sporadic nasopharyngeal carcinomas.
在上呼吸消化道癌中已检测到人类乳头瘤病毒（HPV）DNA。然而，该主题的研究结果显示出相当大的差异，HPV与头颈部鳞状细胞癌之间的因果关系仍有待确定。在鼻咽癌病变中始终能检测到爱泼斯坦-巴尔病毒（EBV），但对其与头颈部其他癌症的关联了解甚少。本研究旨在探讨HPV和EBV在上呼吸消化道上皮癌中的作用。
研究材料包括79份头颈部上皮癌的冷冻活检样本。从冷冻活检样本中提取DNA，使用Southern印迹杂交（SBH）和聚合酶链反应（PCR）进行分析，以检测HPV DNA。
在低严谨条件下，通过SBH在13个样本（16.5%）中检测到HPV DNA，使用靶向HPV L1区域的通用引物通过PCR在3个样本（3.8%）中检测到HPV DNA。HPV似乎对唇癌有亲和力：6个样本中有4个（66.7%）HPV DNA呈阳性。从唇和口腔上皮到喉区域，HPV的检测率逐渐降低。在SBH中，任何活检样本中均未发现EBV DNA。
HPV似乎参与了头颈部上皮的多因素致癌过程，但这种关联不如在生殖系统癌症中那么明显。结果表明EBV与散发性鼻咽癌无关。
