Umetsu Kazuo, Tanaka Masashi, Yuasa Isao, Adachi Noboru, Miyoshi Aya, Kashimura Seiichi, Park Kyung Sook, Wei Yau-Huei, Watanabe Gotaro, Osawa Motoki
Department of Experimental and Forensic Pathology, Faculty of Medicine, Yamagata University, Yamagata, Japan.
Electrophoresis. 2005 Jan;26(1):91-8. doi: 10.1002/elps.200406129.
We present a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the haplogroup-diagnostic mitochondrial single-nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup-specific mtSNPs in the coding region by using four 9-multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45 relevant haplogroups. This multiplex APLP analysis of coding-region mtSNPs for haplogrouping is especially useful not only for molecular phylogenetic studies but also for large-scale association studies due to its rapid and economical nature. This is the first panel of mtSNPs in the coding region to be used for haplogrouping of East Asian populations.
我们提出了一种可靠、快速且经济的多重扩增产物长度多态性（APLP）方法，用于分析东亚人群中用于单倍群诊断的线粒体单核苷酸多态性（mtSNPs）。通过使用四个9重聚合酶链反应（PCR）并随后进行电泳，仅检测编码区的36个单倍群特异性mtSNPs，我们就能够将来自日本、韩国、中国和德国8个群体的1815名个体安全地分配到45个相关单倍群中。这种用于单倍群分类的编码区mtSNPs多重APLP分析不仅因其快速和经济的特性而对分子系统发育研究特别有用，而且对大规模关联研究也特别有用。这是首个用于东亚人群单倍群分类的编码区mtSNPs面板。
