Milićević Novica M, Nohroudi Klaus, Milićević Zivana, Westermann Jürgen
Faculty of Medicine, Institute of Histology and Embryology, University of Belgrade, Visegradska 26, Belgrade YU-11000, Serbia and Montenegro.
Immunol Invest. 2004;33(4):439-52. doi: 10.1081/imm-200038682.
Adhesion molecules expressed on surface membranes of lymphocytes and other leukocytes enable their entry into the lymphoid and other tissues. However, little is known about molecules that govern the transit of leukocytes through the parenchyma of lymphoid organs proper. We show that in comparison to blood leukocytes, the corresponding cells isolated from lymphoid organs, i.e., lymph nodes and spleen, have a significantly augmented expression of certain surface molecules. The helper and cytotoxic subsets of T cells, as well as B cells, display the increased expression of CD44, ICAM-1 and LFA-1, whereas B cells additionally show the augmented expression of MHC class II. In comparison with blood monocytes, splenic monocytes show the increased expression of ICAM-1 and MHC class II molecules. When compared with blood NK cells, splenic NK cells only show the increased expression of ICAM-1. The molecules, which we show to be up regulated upon the entry of leukocytes into lymphoid organs, could be involved in their retention within the tissue via cell-cell or cell-extracellular matrix interactions and in control of their transit through lymphoid tissues.
淋巴细胞和其他白细胞表面膜上表达的黏附分子使其能够进入淋巴组织和其他组织。然而，对于调控白细胞穿过淋巴器官实质的分子，人们了解甚少。我们发现，与血液中的白细胞相比，从淋巴器官（即淋巴结和脾脏）分离出的相应细胞某些表面分子的表达显著增加。T细胞的辅助性和细胞毒性亚群以及B细胞，CD44、ICAM-1和LFA-1的表达增加，而B细胞还显示出MHC II类分子的表达增加。与血液中的单核细胞相比，脾脏中的单核细胞ICAM-1和MHC II类分子的表达增加。与血液中的NK细胞相比，脾脏中的NK细胞仅显示ICAM-1的表达增加。我们发现白细胞进入淋巴器官时上调的这些分子，可能通过细胞间或细胞与细胞外基质的相互作用参与其在组织中的滞留，并控制其在淋巴组织中的转运。
