Klonz A, Wonigeit K, Pabst R, Westermann J
Center of Anatomy, Medical School of Hannover, Germany.
Scand J Immunol. 1996 Nov;44(5):461-9. doi: 10.1046/j.1365-3083.1996.d01-334.x.
To leave the blood, leucocytes marginate to the vessel wall. Granulocytes thereby form the so-called marginal pool. It is unclear to what extent such a second intravascular compartment also exists for lymphocytes subsets, NK-cells and monocytes. Samples of the peripheral blood and the marginal pool of the LEW rat were analysed by flow cytometry. In the marginal pool the percentage of granulocytes and monocytes was significantly higher compared to that of the peripheral blood, and the proportion of 'naive' T and B lymphocytes was decreased. The expression of LFA-1 was higher on all leucocyte subsets of the marginal pool except the granulocytes, whereas no differences were seen for the expression of other adhesion molecules (alpha 4-integrins, ICAM-1, CD2, L-selectin, and CD44). In addition, splenectomy influenced the cellular composition of peripheral blood and marginal pool differently and, after injection of blood leucocytes, these cells were found in both compartments showing its characteristic cellular composition. Thus, not only granulocytes, but also B and T lymphocyte subsets, NK-cells and monocytes form a second distinct intravascular compartment. This marginal pool probably influences the cellular composition of leucocyte subsets available for entry into the tissues.
为了离开血液，白细胞会边缘化至血管壁。粒细胞由此形成所谓的边缘池。目前尚不清楚淋巴细胞亚群、自然杀伤细胞（NK细胞）和单核细胞在多大程度上也存在这样一个血管内的第二部分。通过流式细胞术分析了LEW大鼠外周血和边缘池的样本。与外周血相比，边缘池中粒细胞和单核细胞的百分比显著更高，而“幼稚”T淋巴细胞和B淋巴细胞的比例则降低。除粒细胞外，边缘池所有白细胞亚群上淋巴细胞功能相关抗原-1（LFA-1）的表达更高，而其他黏附分子（α4整合素、细胞间黏附分子-1、CD2、淋巴细胞归巢受体L-选择素和CD44）的表达未见差异。此外，脾切除术对外周血和边缘池的细胞组成影响不同，注射血液白细胞后，这些细胞在两个部分均被发现，呈现出其特征性的细胞组成。因此，不仅粒细胞，而且B淋巴细胞亚群、T淋巴细胞亚群、NK细胞和单核细胞都形成了一个独特的血管内第二部分。这个边缘池可能会影响可进入组织的白细胞亚群的细胞组成。
