Ryuko K, Iwanari O, Nakayama S, Kitao M, Karino K, Endo J
Department of Obstetrics and Gynecology, Shimane Medical University, Izumo, Japan.
Cancer. 1992 May 1;69(9):2361-7. doi: 10.1002/1097-0142(19920501)69:9<2361::aid-cncr2820690926>3.0.co;2-q.
The assay conditions needed for an immunoradiometric competitive inhibition assay of sera in healthy women were studied using the monoclonal antibody TKH2, which is known to recognize specifically sialosyl-alpha 2,6-GalNAc alpha 1-0-serine/threonine (S-Tn) antigen, a mucinous cancer-related antigen. Stable results were obtained with an incubation time of 1.5 hours at room temperature. The intra-assay and inter-assay coefficients of variation were 3.27% and 3.07%, respectively. The mean (+/- standard deviation [SD]) levels of serum S-Tn in 602 healthy women was 21.2 U/ml (+/- 8.4 U/ml). Values showed a normal logarithmic distribution. Although slightly higher levels were seen in postmenopausal compared with premenopausal women, the differences were not significant. The cutoff value of 41 U/ml was determined from data obtained in 602 healthy women; higher levels were observed in only 2%. Serum S-Tn levels were not strongly influenced by Lewis or ABO (H) blood type, smoking, pregnancy, parturition, or phase of menstrual cycle. The use of the S-Tn antigen as a tumor marker for various gynecologic cancers requires study.
利用单克隆抗体TKH2研究了健康女性血清免疫放射竞争抑制试验所需的检测条件，已知该单克隆抗体可特异性识别唾液酸-α2,6-GalNAcα1-0-丝氨酸/苏氨酸（S-Tn）抗原，这是一种与黏液性癌相关的抗原。在室温下孵育1.5小时可获得稳定结果。批内和批间变异系数分别为3.27%和3.07%。602名健康女性血清S-Tn的平均（±标准差[SD]）水平为21.2 U/ml（±8.4 U/ml）。数值呈正态对数分布。尽管绝经后女性的水平略高于绝经前女性，但差异不显著。根据602名健康女性的数据确定临界值为41 U/ml；仅2%的人观察到更高水平。血清S-Tn水平不受Lewis或ABO（H）血型、吸烟、妊娠、分娩或月经周期阶段的强烈影响。将S-Tn抗原用作各种妇科癌症的肿瘤标志物需要进行研究。
