Imura H, Endo J, Ohkura H, Ishii M, Ariyoshi Y, Abe O, Masamune O, Nishimoto Y, Fukushi Y, Orikasa S
Gan To Kagaku Ryoho. 1987 May;14(5 Pt 1):1315-21.
We describe a solid-phase immunoradiometric sandwich assay method for a new tumor marker, sialyl SSEA-1 (sialyl LeX-i), using RIA kits (prepared by Otsuka Assay Laboratories). The assay required only duplicate 20-microliter samples, and the concentration of sialyl SSEA-1 antigen in serum was determined with reference to a standard curve ranging from 0 to 224 arbitrary unit/ml. The intra- and inter-assay reproducibilities and analytical recovery of antigen were excellent. The cut-off value (38 unit/ml) was obtained as mean + 2SD, which was calculated from the antigen concentration in sera from 1,105 healthy individuals. Also described are the alterations of normal values by age, sex, and Lewis blood group status.
我们描述了一种使用由大冢检测实验室制备的放射免疫分析试剂盒，针对新肿瘤标志物唾液酸化SSEA-1（唾液酸化LeX-i）的固相免疫放射分析夹心法。该分析仅需20微升的双份样本，并参照0至224任意单位/毫升的标准曲线测定血清中唾液酸化SSEA-1抗原的浓度。抗原的批内和批间重复性以及分析回收率都非常出色。临界值（38单位/毫升）是通过1105名健康个体血清中的抗原浓度计算得出的均值加2个标准差。文中还描述了年龄、性别和Lewis血型状态对正常值的影响。
