Pesillo S Anna, Freeman Lisa M, Rush John E
Department of Clinical Sciences, School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA.
Am J Vet Res. 2004 Dec;65(12):1621-4. doi: 10.2460/ajvr.2004.65.1621.
To determine plasma malondialdehyde (MDA) and serum vitamin E concentrations in dogs with immune-mediated hemolytic anemia (IMHA) and healthy control dogs.
Serum and plasma samples from 36 dogs with IMHA and 40 healthy control dogs.
Blood samples were collected from all study dogs. Plasma MDA concentrations were measured by use of a commercial colorimetric assay, and serum vitamin E concentrations (alpha-, gamma, and delta-tocopherol concentrations) were measured via high-performance liquid chromatography.
Plasma MDA concentrations were significantly higher in the dogs with IMHA than in the control dogs. Compared with control dogs, serum alpha-, gamma-, and &tocopherol concentrations were significantly lower in the IMHA-affected dogs.
Results indicated a state of oxidative stress and reduced antioxidant reserve in dogs with IMHA; this finding provides support for further investigation of the potential benefits of antioxidant treatment in dogs with this disease.
测定免疫介导性溶血性贫血（IMHA）犬和健康对照犬的血浆丙二醛（MDA）及血清维生素E浓度。
36只IMHA犬和40只健康对照犬的血清及血浆样本。
采集所有研究犬的血样。采用商业比色法测定血浆MDA浓度，通过高效液相色谱法测定血清维生素E浓度（α-、γ-和δ-生育酚浓度）。
IMHA犬的血浆MDA浓度显著高于对照犬。与对照犬相比，IMHA患犬的血清α-、γ-和δ-生育酚浓度显著降低。
结果表明IMHA犬存在氧化应激状态且抗氧化储备降低；这一发现为进一步研究抗氧化治疗对该病犬的潜在益处提供了支持。
