肝细胞癌患者非癌肝残余组织中缝隙连接基因连接蛋白信使核糖核酸的表达与术后复发相关吗？

Do the expressions of gap junction gene connexin messenger RNA in noncancerous liver remnants of patients with hepatocellular carcinoma correlate with postoperative recurrences?

作者信息

Sheen I-Shyan, Jeng Kuo-Shyang, Shih Shou-Chuan, Kao Chin-Roa, Wang Po-Chuan, Chen Chih-Zen, Chang Wen-Hsing, Wang Horng-Yuan, Shyung Li-Rung

机构信息

Division of Hepatogastroenterology, Chang Gung Memorial Hospital, Taipei, Taiwan, China.

出版信息

World J Gastroenterol. 2005 Jan 14;11(2):171-5. doi: 10.3748/wjg.v11.i2.171.

DOI:10.3748/wjg.v11.i2.171

PMID:15633210

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4205396/

Abstract

AIM

To investigate whether the changes of gap junction gene connexin messenger RNA in the noncancerous liver tissue of patients with hepatocellular carcinoma (HCC) could play a significant role in its postresection recurrence.

METHODS

Seventy-nine consecutive patients having undergone curative resection for HCC entered this study. Using a reverse-transcription polymerase chain reaction (RT-PCR)-based assay, connexin (Cx) 26, connexin (Cx) 32 and connexin (Cx) 43 mRNAs were determined prospectively in noncancerous liver tissues from these 79 patients and in the liver tissues from 15 controls. The correlations between connexin mRNA expression and the clinicopathological variables and outcomes (tumor recurrence and recurrence related mortality) were studied.

RESULTS

Compared with liver tissues of control patients, the expression of Cx 32 mRNA in noncancerous liver tissues was significantly lower (mean: 0.715 vs control 1.225, P<0.01), whereas the decreased Cx 26 mRNA (mean: 0.700 vs of control 1.205, P>0.05) and increased Cx 43 mRNA (mean: 0.241 vs control 0.100, P>0.05) had no statistical significance. We defined the value of Cx 32 mRNA or Cx 26 mRNA below 0.800 as a lower value. By multivariate analysis for noncancerous livers, a lower value of Cx 32 mRNA correlated significantly with a risk of HCC recurrence and recurrence-related mortality. The lower value of Cx 26 mRNA did not correlate with recurrence and mortality. The increased value of Cx43 mRNA also did not correlate with postoperative recurrence and recurrence-related mortality. By multivariate analysis, other significant predictors of HCC recurrence included vascular permeation, cellular dedifferentiation, and less encapsulation. The other significant parameter of recurrence related mortality was vascular permeation.

CONCLUSION

The decreased expression of Cx 32 mRNA in noncancerous liver tissues plays a significant role in the prediction of postoperative recurrence of HCC.

摘要

目的

探讨肝细胞癌（HCC）患者非癌肝组织中间隙连接基因连接蛋白信使核糖核酸的变化是否在其切除术后复发中起重要作用。

方法

79例连续接受HCC根治性切除的患者进入本研究。采用基于逆转录聚合酶链反应（RT-PCR）的检测方法，前瞻性地测定这79例患者非癌肝组织及15例对照者肝组织中连接蛋白（Cx）26、连接蛋白（Cx）32和连接蛋白（Cx）43信使核糖核酸。研究连接蛋白信使核糖核酸表达与临床病理变量及预后（肿瘤复发和复发相关死亡率）之间的相关性。

结果

与对照患者的肝组织相比，非癌肝组织中Cx 32信使核糖核酸的表达显著降低（平均值：0.715对对照组1.225，P<0.01），而Cx 26信使核糖核酸的降低（平均值：0.700对对照组1.205，P>0.05）和Cx 43信使核糖核酸的升高（平均值：0.241对对照组0.100，P>0.05）无统计学意义。我们将Cx 32信使核糖核酸或Cx 26信使核糖核酸低于0.800的值定义为较低值。对非癌肝组织进行多因素分析，Cx 32信使核糖核酸较低值与HCC复发风险及复发相关死亡率显著相关。Cx 26信使核糖核酸较低值与复发及死亡率无关。Cx43信使核糖核酸升高值也与术后复发及复发相关死亡率无关。多因素分析显示，HCC复发的其他重要预测因素包括血管浸润、细胞去分化和包膜不完整。复发相关死亡率的另一个重要参数是血管浸润。