间隙连接蛋白32在慢性肝病中的表达
Expression of gap junction protein connexin 32 in chronic liver diseases.
作者信息
Yamaoka K, Nouchi T, Kohashi T, Marumo F, Sato C
机构信息
Department of Internal Medicine, Hokushin General Hospital, Nagano, Japan.
出版信息
Liver. 2000 Apr;20(2):104-7. doi: 10.1034/j.1600-0676.2000.020002104.x.
BACKGROUND
Gap junctions contain intercellular channels through which contacting cells communicate directly. The expression of connexin 32, a major gap junction protein in the liver, during the progression of chronic liver diseases has not yet been clarified.
METHODS
Immunohistochemical staining was performed using anti-connexin 32 antibody on 6 specimens of normal human liver, 7 of chronic viral hepatitis, and 7 of liver cirrhosis.
RESULTS
The number of gap junction plaques in chronic viral hepatitis and liver cirrhosis was significantly smaller than that in normal liver (10350+/-2180 and 7550+/-3040 vs 22560+/-3700 spots/mm2, p<0.01). The number of gap junction plaques tended to be lower in liver cirrhosis than in chronic viral hepatitis.
CONCLUSION
These results suggest that in chronic liver diseases impaired intercellular communication between hepatocytes occurs.
背景
缝隙连接包含细胞间通道，相邻细胞可通过该通道直接进行通讯。连接蛋白32是肝脏中主要的缝隙连接蛋白，其在慢性肝病进展过程中的表达情况尚未明确。
方法
使用抗连接蛋白32抗体对6例正常人类肝脏标本、7例慢性病毒性肝炎标本和7例肝硬化标本进行免疫组织化学染色。
结果
慢性病毒性肝炎和肝硬化中缝隙连接斑的数量显著少于正常肝脏（分别为10350±2180和7550±3040个斑点/mm²，而正常肝脏为22560±3700个斑点/mm²，p<0.01）。肝硬化中缝隙连接斑的数量往往低于慢性病毒性肝炎。
结论
这些结果表明，在慢性肝病中，肝细胞之间的细胞间通讯受损。
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