Ho Y K, Kung M P, Bardos T J
Department of Medicinal Chemistry, State University of New York at Buffalo, Buffalo, NY 14214, USA.
Biochem Biophys Res Commun. 1976 Dec 20;73(4):903-10. doi: 10.1016/0006-291x(76)90207-2.
A series of oligomers of cytidylic acid were prepared and partially (6-9% of the bases) thiolated in the 5 positions. The modified oligomers showed increasing inhibition with increasing chain length of both the DNA polymerase-alpha from regenerating rat liver and the DNA-dependent RNA polymerase of E. coli, but the minimum chain length for observable inhibitory activity was 5 nucleotide units for the DNA polymerase-alpha and 16 units for the RNA polymerase.
制备了一系列胞苷酸寡聚物，并在5位进行了部分（6 - 9%的碱基）硫醇化修饰。修饰后的寡聚物对再生大鼠肝脏中的DNA聚合酶α和大肠杆菌的DNA依赖性RNA聚合酶的抑制作用均随链长增加而增强，但对于DNA聚合酶α，可观察到抑制活性的最小链长为5个核苷酸单位，对于RNA聚合酶则为16个单位。
