Li Jia-Qi, Liu Xiao-Hong, Wang Chong, Chen Zan-Mou, Wu Qiu-Hao, Zhang Xi-Quan, Liu De-Wu, Chen Yao-Sheng
College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Yi Chuan Xue Bao. 2004 Dec;31(12):1361-8.
With F2 design, 16 Chinese Lantang sows crossbred with eight highly improved Landrace boars to establish a resource population including 40 F1 sows, eight F1 boars, and 232 F2 pigs. Genetic analysis of the resource population showed that the 32 performance traits displayed some degree of variation, and coefficients of variation of the majority of economic traits exceeded 10%. The variance component analysis revealed that the ratios of additive genetic variance to phenotypic variance of the majority of economic traits were high. Of the 22 microsatellite DNA markers, only 12 microsatellite DNA markers are polymorphisic in this populations. The average heterozygosity of these markers and PIC were 0.53 and 0.46 respectively. The results showed that these markers can provide enough information for QTL mapping. In conclusion,the F2 pigs were sufficiently segregated, and were capable of serving as a resource population for QTL mapping.
采用F2设计,将16头中国蓝塘母猪与8头高度改良的长白公猪杂交,建立了一个资源群体,包括40头F1母猪、8头F1公猪和232头F2猪。对该资源群体的遗传分析表明,32个性状表现出一定程度的变异,大多数经济性状的变异系数超过10%。方差成分分析表明,大多数经济性状的加性遗传方差与表型方差的比值较高。在22个微卫星DNA标记中,该群体中只有12个微卫星DNA标记具有多态性。这些标记的平均杂合度和多态信息含量分别为0.53和0.46。结果表明,这些标记可为QTL定位提供足够的信息。综上所述,F2猪分离充分,可作为QTL定位的资源群体。