Rzedzicki J, Boś M, Kolasa A
Institute of Infectious and Invasive Diseases, Faculty of Veterinary Medicine, University of Agriculture in Lublin, Akademicka 12, 20-033 Lublin, Poland.
Pol J Vet Sci. 2004;7(4):267-74.
Variety of traits important in diagnostics and epidemiology of pathogenic microorganisms may change due to antibiotics. Movement ability, that is characteristic for every serovar except from Salmonella Gallinarum-Pullorum, is important to salmonellas. In own experiments using semi-fluid MSRV medium, it was found that a decrease in salmonella sensibility to selected antibiotics and chemiotherapeutics due to passage might lead to weakening of its movement ability. Movement ability of all strains (S. Enteritidis, S. Dublin, S. Typhimurium) after passage with amoxycillin, neomycin, colistin and enrofloxacin became weakened as compared to results achieved before passage. The strongest inhibition of movement ability was most often observed in strains after passage on medium with colistin. It seems to be associated with the action mechanism of the antibiotic. Colistin injuries cellular membranes, where flagella (active motoric organ of Salmonella) are anchored. Appearance of drug-resistance as a result of passage at the presence of antibiotics may cause variability of biochemical properties of Salmonella rods and leads to weakening of movement ability of ciliated Salmonella.
致病性微生物诊断和流行病学中重要的各种特性可能会因抗生素而改变。运动能力对沙门氏菌很重要，除鸡白痢沙门氏菌外，每种血清型都具有运动能力。在我们使用半流体MSRV培养基进行的实验中，发现沙门氏菌由于传代而对选定抗生素和化学治疗剂的敏感性降低可能会导致其运动能力减弱。与传代前的结果相比，所有菌株（肠炎沙门氏菌、都柏林沙门氏菌、鼠伤寒沙门氏菌）在经过阿莫西林、新霉素、黏菌素和恩诺沙星传代后，其运动能力均变弱。在含有黏菌素的培养基上传代后的菌株中，最常观察到运动能力受到最强抑制。这似乎与抗生素的作用机制有关。黏菌素会损伤细胞膜，而鞭毛（沙门氏菌的活性运动器官）就附着在细胞膜上。在有抗生素存在的情况下传代导致的耐药性出现可能会引起沙门氏菌杆状菌生化特性的变化，并导致有鞭毛的沙门氏菌运动能力减弱。
