通过实时荧光定量聚合酶链反应检测对接触感染非洲猪瘟的猪进行临床前诊断。
Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay.
作者信息
Zsak L, Borca M V, Risatti G R, Zsak A, French R A, Lu Z, Kutish G F, Neilan J G, Callahan J D, Nelson W M, Rock D L
机构信息
Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944-0848, USA.
出版信息
J Clin Microbiol. 2005 Jan;43(1):112-9. doi: 10.1128/JCM.43.1.112-119.2005.
Abstract
A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.
摘要
开发了一种用于非洲猪瘟病毒（ASFV）的荧光探针水解（TaqMan）PCR检测方法，并在实验感染的猪中进行了评估。这种灵敏且特异的一步单管检测方法可在2小时或更短时间内完成，能在临床疾病发作前2至4天检测扁桃体刮取样本中的病毒DNA。因此，该检测方法可用于非洲猪瘟的临床前诊断以及疾病爆发的监测和/或应急管理。
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