用于临床样本中非洲猪瘟病毒常规诊断的高灵敏度聚合酶链式反应检测法。
Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples.
作者信息
Agüero M, Fernández J, Romero L, Sánchez Mascaraque C, Arias M, Sánchez-Vizcaíno J M
机构信息
Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain.
出版信息
J Clin Microbiol. 2003 Sep;41(9):4431-4. doi: 10.1128/JCM.41.9.4431-4434.2003.
Abstract
This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.
摘要
这项工作提供了一种新颖、高度灵敏的热启动聚合酶链反应（PCR）方法，用于快速、特异性地检测非洲猪瘟病毒（ASFV），该方法可作为监测、控制和根除计划中ASFV的常规诊断测试。还开发了一种基于限制性内切酶分析的该方法特异性确证试验。
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