通过便携式实时逆转录聚合酶链反应检测法快速检测经典猪瘟病毒
Rapid detection of classical swine fever virus by a portable real-time reverse transcriptase PCR assay.
作者信息
Risatti G R, Callahan J D, Nelson W M, Borca M V
机构信息
Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York 11944, USA.
出版信息
J Clin Microbiol. 2003 Jan;41(1):500-5. doi: 10.1128/JCM.41.1.500-505.2003.
Abstract
A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR assay for classical swine fever virus (CSFV) was developed and evaluated in experimentally infected swine. The assay detected CSFV, representing different phylogenetic groupings, but did not amplify viral RNA from related pestiviruses. The assay met or exceeded the sensitivity (1 to 100 50% tissue culture infective doses per ml) of viral cultures of samples from experimentally infected animals. Viral RNA was detected in nasal and tonsil scraping samples 2 to 4 days prior to the onset of clinical disease. The assay can be performed in 2 h or less, thus providing a rapid method for the diagnosis of classical swine fever.
摘要
开发了一种用于检测经典猪瘟病毒（CSFV）的荧光探针水解（TaqMan）逆转录聚合酶链反应检测方法，并在实验感染的猪中进行了评估。该检测方法能检测出代表不同系统发育分组的CSFV，但不能扩增相关瘟病毒的病毒RNA。该检测方法达到或超过了实验感染动物样本病毒培养的灵敏度（每毫升1至100个50%组织培养感染剂量）。在临床疾病发作前2至4天，在鼻腔和扁桃体刮取样本中检测到病毒RNA。该检测方法可在2小时或更短时间内完成，从而为经典猪瘟的诊断提供了一种快速方法。
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