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J Clin Microbiol. 2005 Jan;43(1):439-41. doi: 10.1128/JCM.43.1.439-441.2005.
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Protocol for the accelerated detection of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae strains from blood cultures.
Eur J Clin Microbiol Infect Dis. 2004 Mar;23(3):200-2. doi: 10.1007/s10096-003-1086-0. Epub 2004 Feb 7.
2
Utility of NCCLS guidelines for identifying extended-spectrum beta-lactamases in non-Escherichia coli and Non-Klebsiella spp. of Enterobacteriaceae.美国国家临床实验室标准委员会（NCCLS）指南在鉴定肠杆菌科非大肠杆菌和非克雷伯菌属中广谱β-内酰胺酶的实用性。
J Clin Microbiol. 2004 Jan;42(1):294-8. doi: 10.1128/JCM.42.1.294-298.2004.
3
The importance of appropriate initial antibiotic therapy for hospital-acquired infections.
Am J Med. 2003 Nov;115(7):582-4. doi: 10.1016/j.amjmed.2003.09.002.
4
Inappropriate initial antimicrobial therapy and its effect on survival in a clinical trial of immunomodulating therapy for severe sepsis.在一项针对严重脓毒症免疫调节治疗的临床试验中，不恰当的初始抗菌治疗及其对生存的影响。
Am J Med. 2003 Nov;115(7):529-35. doi: 10.1016/j.amjmed.2003.07.005.
5
Occurrence and phenotypic characteristics of extended-spectrum beta-lactamases among members of the family Enterobacteriaceae at the Tel-Aviv Medical Center (Israel) and evaluation of diagnostic tests.以色列特拉维夫医疗中心肠杆菌科细菌中广谱β-内酰胺酶的发生率及表型特征，以及诊断试验的评估
J Clin Microbiol. 2003 Jan;41(1):155-8. doi: 10.1128/JCM.41.1.155-158.2003.
6
Prevalence of extended spectrum beta-lactamase (ESBL)-producing clinical isolates in the Asia-Pacific region and South Africa: regional results from SENTRY Antimicrobial Surveillance Program (1998-99).亚太地区和南非产超广谱β-内酰胺酶（ESBL）临床分离株的流行情况：哨兵抗菌监测计划（1998 - 1999年）的区域结果
Diagn Microbiol Infect Dis. 2002 Mar;42(3):193-8. doi: 10.1016/s0732-8893(01)00353-4.
7
Variations in the prevalence of strains expressing an extended-spectrum beta-lactamase phenotype and characterization of isolates from Europe, the Americas, and the Western Pacific region.表达超广谱β-内酰胺酶表型菌株的流行率变化以及来自欧洲、美洲和西太平洋地区的分离株特征
Clin Infect Dis. 2001 May 15;32 Suppl 2:S94-103. doi: 10.1086/320182.
8
Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae: risk factors for infection and impact of resistance on outcomes.产超广谱β-内酰胺酶的大肠埃希菌和肺炎克雷伯菌：感染的危险因素及耐药性对结局的影响
Clin Infect Dis. 2001 Apr 15;32(8):1162-71. doi: 10.1086/319757. Epub 2001 Mar 26.
9
Detection of extended-spectrum beta-lactamases in klebsiellae with the Oxoid combination disk method.用奥克托克斯组合纸片法检测克雷伯菌属中的超广谱β-内酰胺酶
J Clin Microbiol. 2000 Nov;38(11):4228-32. doi: 10.1128/JCM.38.11.4228-4232.2000.
10
Antimicrobial resistance in Enterobacteriaceae in Brooklyn, NY: epidemiology and relation to antibiotic usage patterns.纽约布鲁克林肠杆菌科细菌的抗菌药物耐药性：流行病学及其与抗生素使用模式的关系。
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评估一种用于从阳性血培养物中检测产超广谱β-内酰胺酶革兰氏阴性杆菌的加速方案。

Evaluation of an accelerated protocol for detection of extended-spectrum beta-lactamase-producing gram-negative bacilli from positive blood cultures.

作者信息

Navon-Venezia Shiri, Leavitt Azita, Ben-Ami Ronen, Aharoni Yuval, Schwaber Mitchell J, Schwartz David, Carmeli Yehuda

机构信息

Molecular Epidemiology Laboratory, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, 6 Weizman St., Tel-Aviv 64239, Israel.

出版信息

J Clin Microbiol. 2005 Jan;43(1):439-41. doi: 10.1128/JCM.43.1.439-441.2005.

DOI:10.1128/JCM.43.1.439-441.2005
PMID:15635009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC540179/
Abstract

We evaluated a protocol for the accelerated detection of extended-spectrum beta-lactamases (ESBLs) in gram-negative bloodstream pathogens. Two hundred eighty-three blood culture bottles were subjected to direct ESBL testing by inoculating samples directly from blood culture bottles onto agar plates containing cefotaxime and ceftazidime disks, with and without clavulanate. Standard ESBL testing in accordance with the NCCLS guidelines after subculturing on agar plates was performed in parallel. Results of the direct ESBL testing were reported 2.3 days sooner and were comparable to those of the standard NCCLS method with sensitivity, specificity, and positive and negative predictive values of 100, 98, 94, and 100%, respectively.

摘要

我们评估了一种用于革兰氏阴性血流病原体中广谱β-内酰胺酶（ESBLs）加速检测的方案。将283个血培养瓶直接接种到含有头孢噻肟和头孢他啶纸片（含或不含克拉维酸）的琼脂平板上，进行ESBL直接检测。同时，按照NCCLS指南在琼脂平板上继代培养后进行标准ESBL检测。直接ESBL检测结果比标准方法提前2.3天报告，且与标准NCCLS方法相当，其敏感性、特异性、阳性预测值和阴性预测值分别为100%、98%、94%和100%。