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在重症监护病房使用含抗生素的琼脂来分离产超广谱β-内酰胺酶的微生物。

The use of antibiotic-containing agars for the isolation of extended-spectrum beta-lactamase-producing organisms in intensive care units.

作者信息

Wilson G, McCabe D

机构信息

Department of Medical Microbiology, Stirling Royal Infirmary, Stirling, UK.

出版信息

Clin Microbiol Infect. 2007 Apr;13(4):451-3. doi: 10.1111/j.1469-0691.2006.01667.x.

DOI:10.1111/j.1469-0691.2006.01667.x
PMID:17359334
Abstract

MacConkey agar containing either cefotaxime 1.0 mg/L or ceftazidime 1.0 mg/L was evaluated for use in screening for extended-spectrum beta-lactamase (ESBL)-producing organisms. The media were evaluated using known ESBL-positive and -negative strains and 630 clinical specimens over a 6-month period. All Enterobacteriaceae isolated were identified and screened for ESBL production by phenotypic methods. In total, 14 ESBL-producing organisms were detected in the clinical samples. All known ESBL-positive strains were also detected. The use of both screening plates was required to detect all ESBLs.

摘要

对含有1.0 mg/L头孢噻肟或1.0 mg/L头孢他啶的麦康凯琼脂用于筛选产超广谱β-内酰胺酶(ESBL)微生物进行了评估。在6个月的时间里,使用已知的ESBL阳性和阴性菌株以及630份临床标本对这些培养基进行了评估。对所有分离出的肠杆菌科细菌进行鉴定,并通过表型方法筛选ESBL的产生情况。临床样本中共检测到14株产ESBL微生物。所有已知的ESBL阳性菌株也被检测到。需要使用两种筛选平板才能检测到所有的ESBL。

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