纸片联合法检测产金属β-内酰胺酶菌株时纸片制备的影响
Influence of disk preparation on detection of metallo-beta-lactamase-producing isolates by the combined disk assay.
作者信息
Andrade Soraya S, Picão Renata C, Campana Eloiza H, Nicoletti Adriana G, Pignatari Antônio C C, Gales Ana C
机构信息
Laboratório Especial de Microbiologia Clínica, Universidade Federal de São Paulo, São Paulo, Brazil.
出版信息
J Clin Microbiol. 2007 Jun;45(6):2058-60. doi: 10.1128/JCM.02467-06. Epub 2007 Apr 4.
Abstract
The combined disk assay has been used for detection of metallo-beta-lactamase-producing isolates. We have observed that the size of inhibition zones produced by many beta-lactam/metallo-beta-lactamase inhibitor (IMBL) combinations may differ depending on the way that the combined disks were prepared. Among the 10 beta-lactam/IMBL combinations tested, only the imipenem/EDTA combination produced similar results.
摘要
联合纸片法已用于检测产金属β-内酰胺酶的菌株。我们观察到，许多β-内酰胺/金属β-内酰胺酶抑制剂（IMBL）组合产生的抑菌圈大小可能因联合纸片的制备方式而异。在所测试的10种β-内酰胺/IMBL组合中，只有亚胺培南/EDTA组合产生了相似的结果。
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