Heinemann P, Just W W
Institut für Biochemie I, Universität Heidelberg, Germany.
FEBS Lett. 1992 Mar 30;300(2):179-82. doi: 10.1016/0014-5793(92)80191-i.
In homogeneous of isolated hepatocytes separated by Nycodenz density gradient centrifugation, two peroxisomal populations are identified that differ in buoyant density. Organelles present in a high density fraction (1.22-1.23 g/cm3) represent mature peroxisomes. Vesicles of intermediate density (1.16-1.17 g/cm3) represent mature peroxisomes. Vesicles of intermediate density (1.16-1.17 g/cm3) are present in much lower concentration and seem to play a particular role in the import and distribution of newly synthesized peroxisomal proteins. In a typical pulse-chase experiment with a 7.5 min pulse, peroxisomal acyl-CoA oxidase is first imported into the peroxisomal fraction of intermediate density. After a chase of up to 30 min, the enzyme is found in mature peroxisomes.
在通过 Nycodenz 密度梯度离心分离的均匀分离的肝细胞中，鉴定出两个浮力密度不同的过氧化物酶体群体。存在于高密度级分（1.22 - 1.23 g/cm³）中的细胞器代表成熟的过氧化物酶体。中等密度（1.16 - 1.17 g/cm³）的囊泡代表成熟的过氧化物酶体。中等密度（1.16 - 1.17 g/cm³）的囊泡浓度要低得多，并且似乎在新合成的过氧化物酶体蛋白的导入和分布中起特定作用。在一个典型的 7.5 分钟脉冲追踪实验中，过氧化物酶体酰基辅酶 A 氧化酶首先被导入到中等密度的过氧化物酶体级分中。经过长达 30 分钟的追踪后，该酶在成熟的过氧化物酶体中被发现。
