肠细胞中的信使核糖核酸分选。与编码蛋白的共定位。

Messenger RNA sorting in enterocytes. Co-localization with encoded proteins.

作者信息

Rings E H, Büller H A, de Boer P A, Grand R J, Montgomery R K, Lamers W H, Charles R, Moorman A F

机构信息

Center for Liver and Intestinal Research (CLDO), University of Amsterdam, The Netherlands.

出版信息

FEBS Lett. 1992 Mar 30;300(2):183-7. doi: 10.1016/0014-5793(92)80192-j.

DOI:10.1016/0014-5793(92)80192-j

PMID:1563519

Abstract

This study describes the intracellular compartmentalization of three different mRNAs in the polarized rat fetal enterocyte. They encode proteins that are known to be localized within different regions of the epithelial cell namely (i) the apical, membrane-bound glycoprotein, lactase-phlorizin hydrolase (lactase), (ii) the mitochondrially localized enzyme, carbamoylphosphate synthetase (CPS), and (iii) the cytoplasmically localized enzyme, phosphoenolpyruvate carboxykinase (PEPCK). These mRNAs are found in close proximity to their respective protein products, i.e. the apical membrane, mitochondria and cytoplasm, respectively. The significance of these observations is twofold; (i) they indicate that mRNAs are sorted into specific domains of the cytosol of intestinal epithelial cells; and (ii) they imply the presence of two distinct pathways of mRNA targeting one that allows transport of mRNAs that are translated on ribosomes associated with the rough endoplasmic reticulum (lactase mRNA), and the other that allows sorting of mRNAs that are translated on free polysomes (CPS and PEPCK mRNA).

摘要

本研究描述了三种不同mRNA在极化的大鼠胎儿肠上皮细胞中的细胞内区室化。它们编码的蛋白质已知定位于上皮细胞的不同区域，即（i）顶端膜结合糖蛋白乳糖酶 - 根皮苷水解酶（乳糖酶），（ii）线粒体定位酶氨甲酰磷酸合成酶（CPS），以及（iii）细胞质定位酶磷酸烯醇丙酮酸羧激酶（PEPCK）。这些mRNA分别在与其各自蛋白质产物（即顶端膜、线粒体和细胞质）紧邻的位置被发现。这些观察结果的意义有两方面：（i）它们表明mRNA被分选到肠上皮细胞胞质溶胶的特定区域；（ii）它们意味着存在两种不同的mRNA靶向途径，一种允许与糙面内质网相关的核糖体上翻译的mRNA运输（乳糖酶mRNA），另一种允许游离多聚核糖体上翻译的mRNA分选（CPS和PEPCK mRNA）。