Sano Y, Kageyama M
Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Mol Gen Genet. 1987 Jul;208(3):412-9. doi: 10.1007/BF00328132.
The recA gene of Pseudomonas aeruginosa has been isolated and its nucleotide sequence has been determined. The coding region of the recA gene has 1038 bp specifying 346 amino acids. The recA protein of P. aeruginosa showed a striking homology with that of Escherichia coli except for the carboxy-terminal region both at the nucleotide and amino acid level. The recA+-carrying plasmids restored the UV sensitivity and recombination ability of several rec mutants of P. aeruginosa. The precise location of the recA gene on the chromosome was deduced from the analysis of R' plasmids.
已分离出铜绿假单胞菌的recA基因，并确定了其核苷酸序列。recA基因的编码区有1038个碱基对，编码346个氨基酸。除了羧基末端区域，铜绿假单胞菌的recA蛋白在核苷酸和氨基酸水平上与大肠杆菌的recA蛋白都有显著的同源性。携带recA⁺的质粒恢复了铜绿假单胞菌几个rec突变体的紫外线敏感性和重组能力。通过对R'质粒的分析推断出recA基因在染色体上的精确位置。
