Li Qing-Bo, Sun Xue-Jun, Xu Yi-Zhuang, Yang Li-Min, Zhang Yuan-Fu, Weng Shi-Fu, Shi Jing-Sen, Wu Jin-Guang
The State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing, Peoples Republic of China.
Clin Chem. 2005 Feb;51(2):346-50. doi: 10.1373/clinchem.2004.037986. Epub 2005 Jan 6.
Fourier transform infrared (FT-IR) spectroscopy is an effective tool for investigation of chemical changes at the molecular level. We previously demonstrated that FT-IR spectroscopy can reliably distinguish multiple types of carcinoma from healthy tissue. Because various stomach diseases are common, it is important to explore a noninvasive and rapid method to detect malignancy and gastritis in endoscopic biopsies. Our aim was to classify endoscopic biopsies into healthy, gastritis, and malignancy through the use of FT-IR spectroscopy.
A total of 103 endoscopic samples, including 19 cases of cancer, 35 cases of chronic atrophic gastritis, 29 cases of chronic superficial gastritis, and 20 healthy tissue samples, were obtained at the First Hospital of Xi'an Jiaotong University, China. A modified attenuated total reflectance accessory was linked to a WQD-500 FT-IR spectrometer for biopsy measurement. The spectral characteristics for different types of tissues were correlated with the corresponding pathology results. The gastric biopsies were classified by FT-IR spectroscopy and a discriminant analysis method.
There were significant differences in the FT-IR spectra of four types of gastric biopsies. The discriminant analysis results demonstrated that the sensitivity of FT-IR detection for healthy, superficial gastritis, atrophic gastritis, and gastric cancer was 90%, 90%, 66%, 74%, respectively, which could help satisfy clinical diagnostic requirements.
FT-IR spectroscopy can distinguish disease processes in gastric endoscopic biopsies.
傅里叶变换红外（FT-IR）光谱是一种在分子水平上研究化学变化的有效工具。我们之前证明FT-IR光谱能够可靠地区分多种类型的癌组织与健康组织。由于各种胃部疾病很常见，探索一种非侵入性且快速的方法来检测内镜活检中的恶性肿瘤和胃炎非常重要。我们的目的是通过使用FT-IR光谱将内镜活检样本分为健康、胃炎和恶性肿瘤三类。
在中国西安交通大学第一附属医院共获取了103份内镜样本，包括19例癌症、35例慢性萎缩性胃炎、29例慢性浅表性胃炎和20份健康组织样本。将改良的衰减全反射附件连接到WQD-500傅里叶变换红外光谱仪上以进行活检测量。不同类型组织的光谱特征与相应的病理结果相关联。通过FT-IR光谱和判别分析方法对胃活检样本进行分类。
四种类型的胃活检样本的傅里叶变换红外光谱存在显著差异。判别分析结果表明，FT-IR检测对健康、浅表性胃炎、萎缩性胃炎和胃癌的灵敏度分别为90%、90%、66%、74%，这有助于满足临床诊断需求。
傅里叶变换红外光谱能够区分胃内镜活检中的疾病进程。
