Peeters C C, Evenberg D, Hoogerhout P, Käyhty H, Saarinen L, van Boeckel C A, van der Marel G A, van Boom J H, Poolman J T
Unit for Bacterial Vaccine Development and Pathogenesis Research, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Infect Immun. 1992 May;60(5):1826-33. doi: 10.1128/iai.60.5.1826-1833.1992.
Synthetic oligosaccharides derived from the capsular polysaccharide (PRP) of Haemophilus influenzae type b were conjugated to carrier proteins via a thioether linkage. Conjugates were made of trimeric and tetrameric ribose-ribitol-phosphate and tetanus toxoid or diphtheria toxin. All conjugates elicited anti-PRP antibody responses with an increasing immunoglobulin G/immunoglobulin M ratio in adult mice and monkeys. Trimer conjugates elicited lower anti-PRP antibody responses compared with tetramer conjugates. Adult monkeys responded equally well to the tetrameric oligosaccharide-tetanus toxoid conjugate as to the oligosaccharide-CRM197 conjugate (HbOC), which elicits protective levels of serum antibodies in human infants after two or three injections.
从b型流感嗜血杆菌的荚膜多糖（PRP）衍生而来的合成寡糖通过硫醚键与载体蛋白偶联。偶联物由三聚体和四聚体核糖-核糖醇-磷酸与破伤风类毒素或白喉毒素制成。所有偶联物在成年小鼠和猴子中均引发了抗PRP抗体反应，且免疫球蛋白G/免疫球蛋白M比值不断增加。与四聚体偶联物相比，三聚体偶联物引发的抗PRP抗体反应较低。成年猴子对四聚体寡糖-破伤风类毒素偶联物的反应与对寡糖-CRM197偶联物（HbOC）的反应一样好，后者在人类婴儿注射两到三次后可引发具有保护水平的血清抗体。
