Institute of Biomechanics and Orthopaedics, German Research Center of Elite Sport, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany.
Eur J Appl Physiol. 2010 Oct;110(3):651-7. doi: 10.1007/s00421-010-1529-0. Epub 2010 Jun 11.
The purpose of the study was to investigate whether a relationship between the loading mode of physical activity and serum cartilage oligomeric matrix protein (COMP) concentration exists and whether the lymphatic system contributes to COMP release into the serum. Serum COMP levels were determined in healthy male subjects before, after and at 18 further time points within 7 h at four separate experimental days with four different loading interventions. The loading intervention included high impact running exercise, slow but deep knee bends, and lymphatic drainage of 30 min duration, respectively, and a resting protocol. The serum COMP levels were measured using a commercially available quantitative enzyme-linked immunosorbent assay. An increase (p < 0.001) in serum COMP concentration was detected immediately after 30 min running exercise. Slow but deep knee bends did not cause any significant changes in serum COMP levels. Lymphatic drainage also had no effect on the serum COMP concentration. After 30 min of complete rest the serum COMP level was significantly (p = 0.008) reduced. The elevation of COMP serum concentration seems to depend on the loading mode of the physical activity and to reflect the extrusion of COMP fragments from the impact loaded articular cartilage or synovial fluid.
本研究旨在探讨体力活动的加载方式与血清软骨寡聚基质蛋白（COMP）浓度之间是否存在关系，以及淋巴系统是否有助于 COMP 释放到血清中。在四个不同的实验日的四个单独的加载干预中，分别在健康男性受试者进行高冲击跑步运动、缓慢而深度的膝关节弯曲以及持续 30 分钟的淋巴引流之前、之后以及 7 小时内的另外 18 个时间点测定血清 COMP 水平。使用市售的定量酶联免疫吸附测定法测量血清 COMP 水平。在 30 分钟的跑步运动后，立即检测到血清 COMP 浓度增加（p<0.001）。缓慢而深度的膝关节弯曲不会导致血清 COMP 水平发生任何显著变化。淋巴引流对血清 COMP 浓度也没有影响。完全休息 30 分钟后，血清 COMP 水平显著降低（p=0.008）。COMP 血清浓度的升高似乎取决于体力活动的加载方式，并反映了从受冲击的关节软骨或滑液中挤出的 COMP 片段。
