Orlovskaia O A, Kaminskaia L N, Khotyleva L V
Genetika. 2007 Mar;43(3):363-9.
Cytological analysis of different meiosis stages was performed in F4 hybrids in comparison with the F1 hybrids obtained through crosses between the hexaploid triticale and genome-substitution forms of Aurolata (AABBUU) and Aurosis (AABBS(sh)S(sh)) wheat, in which D genome of common wheat Aurora was substituted for the genomes of Aegilops umbellulata and Ae. sharonensis, respectively. It was demonstrated that in F4 the level of bivalent conjugation was substantially higher than the expected one. However, the value of meiotic index in F4 hybrids was still small, pointing to incomplete process of the meiosis stabilization, specifically, of the stages following the metaphase I. Based on the data of morphological and biochemical analyses of the hybrids produced, the forms of triticale carrying some properties of the genus Aegilops, which were of interest for genetic and breeding studies, were isolated.
对F4杂种不同减数分裂阶段进行了细胞学分析,并与通过六倍体小黑麦与Aurolata(AABBUU)和Aurosis(AABBS(sh)S(sh))小麦的基因组替代形式杂交获得的F1杂种进行了比较,其中普通小麦Aurora的D基因组分别被伞形山羊草和沙龙山羊草的基因组所替代。结果表明,在F4中,二价体配对水平显著高于预期水平。然而,F4杂种的减数分裂指数值仍然较小,表明减数分裂稳定过程不完整,特别是在中期I之后的阶段。根据所产生杂种的形态和生化分析数据,分离出了具有山羊草属某些特性的小黑麦形式,这些特性对遗传和育种研究具有重要意义。