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重铬酸钾对蚕豆根尖细胞致畸效应的研究

[Study on teratogenic effect of potassium dichromate on Vicia faba root tip cells].

作者信息

Qian Xiao-Wei

机构信息

School of Biology and Environment Science, WenZhou Normal College, WenZhou 325 003, China.

出版信息

Yi Chuan. 2004 May;26(3):337-42.

Abstract

We studied the aberrant effects of different concentrations of potassium dichromate on Vicia faba root tip cells. The micronucleus and chromosome aberration assay was conducted to determine the micronucleus rate and chromosome aberration rate of Vicia faba root tip cells induced by potassium dichromate. The result indicated that potassium dichromate could increase the micronucleus rate of Vicia faba root tip cells. Within certain range of concentration the rate of micronucleus was found to be increased with the increase of potassium dichromate concentration,but beyond this range the rate of micronucleus decreased with further increase of potassium dichromate concentration. The potassium dichromate at different concentrations could increase the cell mitosis index. Besides,it also caused various types of chromosome aberration,and the rates of chromosome aberration were always higher than that of the control group. The conclusion of this study was that potassium dichromate has obvious teratogenic effect on Vicia faba root tip cells.

摘要

我们研究了不同浓度重铬酸钾对蚕豆根尖细胞的异常影响。进行了微核和染色体畸变试验,以确定重铬酸钾诱导的蚕豆根尖细胞的微核率和染色体畸变率。结果表明,重铬酸钾可提高蚕豆根尖细胞的微核率。在一定浓度范围内,微核率随重铬酸钾浓度的增加而升高,但超过此范围,微核率随重铬酸钾浓度的进一步增加而降低。不同浓度的重铬酸钾均可提高细胞有丝分裂指数。此外,它还会引起各种类型的染色体畸变,且染色体畸变率总是高于对照组。本研究的结论是,重铬酸钾对蚕豆根尖细胞具有明显的致畸作用。

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