抗真菌活性的赖氨酸调控蛋白型调节因子的突变导致金黄色假单胞菌PA147 - 2在稳定期表型上具有生长优势。
Mutation of a LysR-type regulator of antifungal activity results in a growth advantage in stationary phase phenotype in Pseudomonas aureofaciens PA147-2.
作者信息
Silby Mark W, Giddens Stephen R, Mahanty H Khris
机构信息
Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand.
出版信息
Appl Environ Microbiol. 2005 Jan;71(1):569-73. doi: 10.1128/AEM.71.1.569-573.2005.
Abstract
The growth advantage in stationary phase (GASP) phenotype was shown to be present in two mutants lacking the antifungal phenotype (Af(-) mutants) of Pseudomonas aureofaciens PA147-2. Complementation demonstrated a correlation between GASP and the antifungal defect in one strain but not in the second. Sequence analysis revealed the Af(-) GASP strain had a mutation in a gene (finR) encoding a LysR-type regulator. Antifungal-minus mutants arose in starved cultures, and those aged cultures had increased fitness. Taken together, the results show that there are at least two paths to the GASP phenotype in P. aureofaciens, one of which results in a concomitant loss of the antifungal phenotype.
摘要
研究表明，在缺乏金色假单胞菌PA147 - 2抗真菌表型的两个突变体（Af(-)突变体）中存在稳定期生长优势（GASP）表型。互补实验表明，在一个菌株中GASP与抗真菌缺陷之间存在相关性，但在另一个菌株中则不存在。序列分析显示，Af(-) GASP菌株在编码LysR型调节因子的基因（finR）中发生了突变。在饥饿培养物中出现了抗真菌能力缺失的突变体，并且那些老化培养物的适应性增强。综合来看，结果表明在金色假单胞菌中至少有两条途径可导致GASP表型，其中一条途径会导致抗真菌表型同时丧失。
相似文献
1
Mutation of a LysR-type regulator of antifungal activity results in a growth advantage in stationary phase phenotype in Pseudomonas aureofaciens PA147-2.抗真菌活性的赖氨酸调控蛋白型调节因子的突变导致金黄色假单胞菌PA147 - 2在稳定期表型上具有生长优势。
Appl Environ Microbiol. 2005 Jan;71(1):569-73. doi: 10.1128/AEM.71.1.569-573.2005.
2
Expression of the Pho regulon negatively regulates biofilm formation by Pseudomonas aureofaciens PA147-2.Pho调节子的表达对金黄色假单胞菌PA147-2的生物膜形成具有负调控作用。
Mol Microbiol. 2001 Oct;42(2):415-26. doi: 10.1046/j.1365-2958.2001.02641.x.
3
The importance of recA mutant strains for the study of antifungal genes in Pseudomonas aureofaciens PA147-2.
Can J Microbiol. 2000 Aug;46(8):744-8. doi: 10.1139/w00-053.
4
A GacS deficiency does not affect Pseudomonas chlororaphis PA23 fitness when growing on canola, in aged batch culture or as a biofilm.在油菜籽上生长、在老化的分批培养物中生长或形成生物膜时，GacS缺陷并不影响绿针假单胞菌PA23的适应性。
Can J Microbiol. 2006 Dec;52(12):1177-88. doi: 10.1139/w06-079.
5
RpoS as an intermediate in RsmA-dependent regulation of secondary antifungal metabolites biosynthesis in Pseudomonas sp. M18.RpoS作为RsmA依赖性调控假单胞菌M18中次级抗真菌代谢物生物合成的中间因子。
FEMS Microbiol Lett. 2007 Mar;268(1):81-7. doi: 10.1111/j.1574-6968.2006.00562.x.
6
Prolonged stationary-phase incubation selects for lrp mutations in Escherichia coli K-12.在大肠杆菌K-12中，延长的稳定期培养会筛选出lrp突变体。
J Bacteriol. 2000 Aug;182(15):4361-5. doi: 10.1128/JB.182.15.4361-4365.2000.
7
Identification of a Genetic Locus in Pseudomonas aureofaciens Involved in Fungal Inhibition.鉴定铜绿假单胞菌中参与真菌抑制的遗传基因座。
Appl Environ Microbiol. 1994 Jan;60(1):71-7. doi: 10.1128/aem.60.1.71-77.1994.
8
Competitive growth advantage of nontoxigenic mutants in the stationary phase in archival cultures of pathogenic Vibrio cholerae strains.致病性霍乱弧菌菌株存档培养物中，非产毒突变体在稳定期的竞争性生长优势。
Infect Immun. 2004 Sep;72(9):5478-82. doi: 10.1128/IAI.72.9.5478-5482.2004.
9
Mutations enhancing amino acid catabolism confer a growth advantage in stationary phase.增强氨基酸分解代谢的突变在稳定期赋予生长优势。
J Bacteriol. 1999 Sep;181(18):5800-7. doi: 10.1128/JB.181.18.5800-5807.1999.
10
Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator.铜绿假单胞菌中rpoS基因表达的调控：一个TetR家族调控因子的作用
J Bacteriol. 2001 Jun;183(12):3712-20. doi: 10.1128/JB.183.12.3712-3720.2001.
引用本文的文献
1
LysR-type transcriptional regulator FinR is required for phenazine and pyrrolnitrin biosynthesis in biocontrol Pseudomonas chlororaphis strain G05.LysR 型转录调节因子 FinR 是生防绿针假单胞菌 G05 菌株中吩嗪和吡咯菌素生物合成所必需的。
Appl Microbiol Biotechnol. 2021 Oct;105(20):7825-7839. doi: 10.1007/s00253-021-11600-8. Epub 2021 Sep 25.
2
Nanocalorimetry Reveals the Growth Dynamics of Escherichia coli Cells Undergoing Adaptive Evolution during Long-Term Stationary Phase.纳米量热法揭示了在长期稳定期经历适应性进化的大肠杆菌细胞的生长动态。
Appl Environ Microbiol. 2019 Jul 18;85(15). doi: 10.1128/AEM.00968-19. Print 2019 Aug 1.
3
and emerge as lone survivors following long-term starvation of oral microbiota.并在长期饥饿口腔微生物群后作为孤独的幸存者出现。
Proc Natl Acad Sci U S A. 2019 Apr 23;116(17):8499-8504. doi: 10.1073/pnas.1820594116. Epub 2019 Apr 11.
4
Genomewide Mutational Diversity in Population Evolving in Prolonged Stationary Phase.处于长期稳定期进化的群体中的全基因组突变多样性
mSphere. 2017 May 24;2(3). doi: 10.1128/mSphere.00059-17. eCollection 2017 May-Jun.
5
Starvation induces phenotypic diversification and convergent evolution in Vibrio vulnificus.饥饿诱导创伤弧菌的表型多样化和趋同进化。
PLoS One. 2014 Feb 13;9(2):e88658. doi: 10.1371/journal.pone.0088658. eCollection 2014.
本文引用的文献
1
Contribution of the Global Regulator Gene gacA to Persistence and Dissemination of Pseudomonas fluorescens Biocontrol Strain CHA0 Introduced into Soil Microcosms.全局调控基因 gacA 对荧光假单胞菌生防菌株 CHA0 定殖和扩散于土壤微宇宙中的贡献。
Appl Environ Microbiol. 1994 Jul;60(7):2553-60. doi: 10.1128/aem.60.7.2553-2560.1994.
2
Cloning of Genes Involved in the Synthesis of Pyrrolnitrin from Pseudomonas fluorescens and Role of Pyrrolnitrin Synthesis in Biological Control of Plant Disease.从荧光假单胞菌中克隆涉及吡咯并硝酮合成的基因及其在植物病害生物防治中的作用。
Appl Environ Microbiol. 1994 Jan;60(1):78-85. doi: 10.1128/aem.60.1.78-85.1994.
3
Identification of a Genetic Locus in Pseudomonas aureofaciens Involved in Fungal Inhibition.鉴定铜绿假单胞菌中参与真菌抑制的遗传基因座。
Appl Environ Microbiol. 1994 Jan;60(1):71-7. doi: 10.1128/aem.60.1.71-77.1994.
4
GASP phenotype: presence in enterobacteria and independence of sigmaS in its acquisition.GASP表型：存在于肠道杆菌中且其获得不依赖于σS 。
FEMS Microbiol Lett. 2003 Aug 29;225(2):201-6. doi: 10.1016/S0378-1097(03)00514-7.
5
Repeated introduction of genetically modified Pseudomonas putida WCS358r without intensified effects on the indigenous microflora of field-grown wheat.多次引入转基因恶臭假单胞菌WCS358r对田间种植小麦的本土微生物区系没有强化影响。
Appl Environ Microbiol. 2003 Jun;69(6):3110-8. doi: 10.1128/AEM.69.6.3110-3118.2003.
6
Inactivation of gacS does not affect the competitiveness of Pseudomonas chlororaphis in the Arabidopsis thaliana rhizosphere.gacS基因的失活并不影响绿针假单胞菌在拟南芥根际的竞争力。
Appl Environ Microbiol. 2003 Mar;69(3):1817-26. doi: 10.1128/AEM.69.3.1817-1826.2003.
7
Microbial populations responsible for specific soil suppressiveness to plant pathogens.对植物病原体具有特定土壤抑制作用的微生物种群。
Annu Rev Phytopathol. 2002;40:309-48. doi: 10.1146/annurev.phyto.40.030402.110010. Epub 2002 May 13.
8
Survival of GacS/GacA mutants of the biological control bacterium Pseudomonas aureofaciens 30-84 in the wheat rhizosphere.生防细菌金色假单胞菌30-84的GacS/GacA突变体在小麦根际的存活情况。
Appl Environ Microbiol. 2002 Jul;68(7):3308-14. doi: 10.1128/AEM.68.7.3308-3314.2002.
9
Inactivation of the regulatory gene algU or gacA can affect the ability of biocontrol Pseudomonas fluorescens CHA0 to persist as culturable cells in nonsterile soil.调节基因algU或gacA的失活会影响生防荧光假单胞菌CHA0在非无菌土壤中作为可培养细胞存活的能力。
Appl Environ Microbiol. 2002 Apr;68(4):2085-8. doi: 10.1128/AEM.68.4.2085-2088.2002.
10
Expression of the Pho regulon negatively regulates biofilm formation by Pseudomonas aureofaciens PA147-2.Pho调节子的表达对金黄色假单胞菌PA147-2的生物膜形成具有负调控作用。
Mol Microbiol. 2001 Oct;42(2):415-26. doi: 10.1046/j.1365-2958.2001.02641.x.
Zotero 插件