Mel'gunov V I, Gromov V L
Biokhimiia. 1977 Jan;42(1):34-42.
The uptake of 8-C14-adenine in N. crassa strain Lindegren (+) was studied. The ability of N. crassa cells to uptake adenine from the medium reaches maximum at the very beginning of the logarithmic stage of growth. Adenine enters the mycelium against the concentration gradient. The uptake of adenine is maximal at 25-30 degrees C, pH 4,6-4,8, and adenine concentration in the medium about 2-15X10(-6) M. The entry of adenine into the cells follows normal Michaelis-Menten kinetics, the apparent Km=0.83+/-0.02 micron. The uptake is inhibited at higher concentrations (10(-3)-10(-4) M) of adenine. 2,6-Diaminopurine, hypoxanthine, guanine, 8-azaadenine and 8-azaguanine inhibit the transport of adenine into the cell. Xanthine and cytosine do not affect the uptake of adenine. Adenine taken up into the cell is rapidly metabolized to AMP, ADP and ATP.
研究了粗糙脉孢菌Lindegren（+）菌株对8-C14-腺嘌呤的摄取。粗糙脉孢菌细胞从培养基中摄取腺嘌呤的能力在对数生长期开始时达到最大值。腺嘌呤逆浓度梯度进入菌丝体。腺嘌呤的摄取在25-30摄氏度、pH值4.6-4.8以及培养基中腺嘌呤浓度约为2-15×10（-6）M时最大。腺嘌呤进入细胞遵循正常的米氏动力学，表观Km=0.83±0.02微米。在较高浓度（10（-3）-10（-4）M）的腺嘌呤时摄取受到抑制。2,6-二氨基嘌呤、次黄嘌呤、鸟嘌呤、8-氮杂腺嘌呤和8-氮杂鸟嘌呤抑制腺嘌呤向细胞内的转运。黄嘌呤和胞嘧啶不影响腺嘌呤的摄取。进入细胞的腺嘌呤迅速代谢为AMP、ADP和ATP。
