Kobayashi H, Mori T, Suzuki A, Nishimura T, Nishimoto H, Harada M
Am J Obstet Gynecol. 1979 Jun 1;134(3):255-9. doi: 10.1016/s0002-9378(16)33029-0.
The effects of estrone, estradiol-17beta, estriol, testosterone, progesterone, and 20alpha-dihydroprogesterone on incorporation of 3H-thymidine into cultured lymphocytes from normal men and nonpregnant women in response to allogeneic stimulation was investigated with two-way MLR. A dose-dependent reduction in the mean percent incorporation was observed in the range of progesterone concentration between 2 x 10(3) and 10(4) ng/ml with statistically significant differences from the control cultures. Estradiol-17 beta at 10(4) ng/ml produced a slight but significant inhibition of incorporation. No significant change in incorporation occurred with the other steroids tested. Since the suppressive property of progesterone in phytohemagglutinin-induced lymphocyte culture reported in our previous communication was reproduced by the present investigation in MLR, the possible role of progesterone in immunoregulation during pregnancy is emphasized as has been postulated previously.
采用双向混合淋巴细胞反应（two-way MLR）研究了雌酮、17β-雌二醇、雌三醇、睾酮、孕酮和20α-二氢孕酮对正常男性和未孕女性培养淋巴细胞在同种异体刺激下掺入3H-胸腺嘧啶核苷的影响。在孕酮浓度为2×10³至10⁴ ng/ml范围内，观察到平均掺入百分比呈剂量依赖性降低，与对照培养物相比有统计学显著差异。10⁴ ng/ml的17β-雌二醇对掺入产生了轻微但显著的抑制作用。所测试的其他类固醇未使掺入发生显著变化。由于本研究在MLR中重现了我们先前通讯中报道的孕酮在植物血凝素诱导的淋巴细胞培养中的抑制特性，如先前所假设的那样，强调了孕酮在孕期免疫调节中的可能作用。
