Kim Su-Jin, Lee Eon-Jeong, Song Yung-Sun, Jeong Hyun-Ja, Lee Kang-Min, Kim Hyung-Ryong, Chae Han-Jung, Shin Tae-Yong, Kim Yun-Kyung, Hong Seung-Heon, Kim Hyung-Min
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, South Korea.
J Ethnopharmacol. 2005 Feb 10;97(1):83-8. doi: 10.1016/j.jep.2004.10.018. Epub 2004 Dec 10.
Traditional Korean medicine, Cheongyeolsaseuptang (CYSST) has been widely applied as a treatment of rheumatoid arthritis (RA) in Korea. However, its effect in experimental models remains unknown. Recent reports suggest that in patients with RA, synovial mast cells increase in number and show signs of activation and production of cytokines. In this study, we investigated the effect of CYSST on production of cytokines by activated human mast cell line, HMC-1. When CYSST (1mg/ml) was added, the production of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-8 was significantly inhibited about 37, 33.6, and 48%, respectively on phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated HMC-1 cells. In addition, CYSST inhibited PMA plus A23187-induced activation of nuclear factor-kappaB. These findings may help understanding the mechanism of action of this medicine leading to control activated mast cells on inflammatory condition like RA.
传统韩医清咽漱汤（CYSST）在韩国已被广泛用于治疗类风湿性关节炎（RA）。然而，其在实验模型中的效果尚不清楚。近期报告表明，在RA患者中，滑膜肥大细胞数量增加，并显示出激活迹象以及细胞因子的产生。在本研究中，我们调查了CYSST对激活的人肥大细胞系HMC-1产生细胞因子的影响。当加入CYSST（1mg/ml）时，在佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）加钙离子载体A23187刺激的HMC-1细胞上，肿瘤坏死因子-α、白细胞介素（IL）-6和IL-8的产生分别显著抑制了约37%、33.6%和48%。此外，CYSST抑制了PMA加A23187诱导的核因子-κB激活。这些发现可能有助于理解这种药物的作用机制，从而在类风湿性关节炎等炎症状态下控制激活的肥大细胞。
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