浙贝母碱通过调节HMC-1细胞中NF-κB和丝裂原活化蛋白激酶的磷酸化来抑制促炎细胞因子的产生。
Peimine Inhibits the Production of Proinflammatory Cytokines Through Regulation of the Phosphorylation of NF-κB and MAPKs in HMC-1 Cells.
作者信息
Park Ji Hye, Lee Bina, Kim Hyun Kab, Kim Eun-Young, Kim Jae-Hyun, Min Ju-Hee, Kim Sunkook, Sohn Youngjoo, Jung Hyuk-Sang
机构信息
Department of Anatomy, College of Korean Medicine, Kyung Hee University, Kyungheedae-ro, Dongdaemun-gu, Seoul, Korea.
Multi-Functional Nano/Bio Electronics Laboratory, Kyung Hee University, Gyeonggi-do, Korea.
出版信息
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S359-S364. doi: 10.4103/0973-1296.210173. Epub 2017 Jul 11.
BACKGROUND
Peimine is a major biologically active component of . Peimine was investigated in chronic inflammation response, but it has not been studied in mast cell-related immediate allergic reaction. The present study aimed to evaluate anti-allergic effect of peimine in human mast cell (HMC-1).
MATERIALS AND METHODS
The effect of peimine on cell viability was measured by MTS assay in HMC-1. Histamine release was investigated in rat peritoneal mast cells (RPMCs). Interleukin (IL)-6, IL-8, and tumor necrosis factor-α (TNF-α) expressions were measured by ELISA assay and reverse transcription-polymerase chain reaction. Mitogen-activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-κB) were examined by Western blot. Passive cutaneous anaphylaxis (PCA) reactions were evaluated using Sprague-Dawley (SD) rats.
RESULTS
Peimine inhibited the production of pro-inflammatory cytokines, such as IL-6, IL-8, and TNF-α. Moreover, peimine reduced MAPKs phosphorylation and the nuclear NF-κB expression in PMACI-induced HMC-1. Peimine decreased PCA reactions in rats as well.
CONCLUSION
Our study proved that peimine might be suitable for the treatment of mast cell-derived allergic inflammatory reactions.
SUMMARY
Peimine inhibited the production of pro-inflammatory cytokines, such as IL-6, IL-8, and TNF-αPeimine reduced MAPKs phosphorylation and the nuclear NF-κB expression in PMACI-induced HMC-1Peimine decreased PCA reactions in ratsPeimine has anti-allergic effect through regulation of pro-inflammatory mechanism on mast cell. HMC-1: Human mast cell, MTS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, RPMCs: Rat peritoneal mast cells. IL-6: Interleukin 6, IL-8: Interleukin 8, TNF-α: Tumor necrosis factor-α, MAPKs: Mitogen-activated protein kinases; NF-κB: Nuclear factor-kappaB, PCA: Passive cutaneous anaphylaxis reactions, SD: Sprague-Dawley.
背景
贝母碱是[具体来源]的主要生物活性成分。已对贝母碱在慢性炎症反应中的作用进行了研究，但尚未对其在肥大细胞相关速发型过敏反应中的作用进行研究。本研究旨在评估贝母碱对人肥大细胞（HMC-1）的抗过敏作用。
材料与方法
采用MTS法检测贝母碱对HMC-1细胞活力的影响。在大鼠腹膜肥大细胞（RPMCs）中研究组胺释放情况。采用酶联免疫吸附测定法（ELISA）和逆转录-聚合酶链反应检测白细胞介素（IL）-6、IL-8和肿瘤坏死因子-α（TNF-α）的表达。通过蛋白质免疫印迹法检测丝裂原活化蛋白激酶（MAPKs）和核因子-κB（NF-κB）。使用斯普拉格-道利（SD）大鼠评估被动皮肤过敏反应（PCA）。
结果
贝母碱抑制促炎细胞因子如IL-6、IL-8和TNF-α的产生。此外，贝母碱降低了PMACI诱导的HMC-1中MAPKs磷酸化和核NF-κB表达。贝母碱也降低了大鼠的PCA反应。
结论
我们的研究证明贝母碱可能适用于治疗肥大细胞源性过敏性炎症反应。
总结
贝母碱抑制促炎细胞因子如IL-6、IL-8和TNF-α的产生。贝母碱降低了PMACI诱导的HMC-1中MAPKs磷酸化和核NF-κB表达。贝母碱降低了大鼠的PCA反应。贝母碱通过调节肥大细胞的促炎机制发挥抗过敏作用。HMC-1：人肥大细胞，MTS：3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四唑，RPMCs：大鼠腹膜肥大细胞。IL-6：白细胞介素6，IL-8：白细胞介素8，TNF-α：肿瘤坏死因子-α，MAPKs：丝裂原活化蛋白激酶；NF-κB：核因子-κB，PCA：被动皮肤过敏反应，SD：斯普拉格-道利。
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