用于疾病诊断的比较蛋白质组学分析方法。

Methods of comparative proteomic profiling for disease diagnostics.

作者信息

Steel Laura F, Haab Brian B, Hanash Samir M

机构信息

Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18901, USA.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 5;815(1-2):275-84. doi: 10.1016/j.jchromb.2004.10.072.

DOI:10.1016/j.jchromb.2004.10.072

PMID:15652816

Abstract

The recent development of numerous technologies for proteome analysis holds the promise of new and more precise methods for disease diagnosis. In this review, we provide an overview of some of these technologies including two-dimensional gel electrophoresis (2DE), historically the workhorse of proteomic analysis, as well as some newer approaches such as liquid phase separations combined with mass spectrometry, and protein microarrays. It is evident that each method has its own strengths and weaknesses and no single method will be optimal in all applications. However, the continuing development of innovative strategies for protein separation and analysis is providing a wealth of new tools for multi-dimensional protein profiling that will advance our capabilities in disease diagnostics and our understanding of disease pathology.

摘要

近期蛋白质组分析众多技术的发展为疾病诊断带来了更新、更精确方法的希望。在本综述中，我们概述了其中一些技术，包括二维凝胶电泳（2DE），它在历史上一直是蛋白质组分析的主力技术，以及一些较新的方法，如液相分离与质谱联用，还有蛋白质微阵列。显然，每种方法都有其优缺点，没有一种方法在所有应用中都是最优的。然而，蛋白质分离和分析创新策略的持续发展正在为多维蛋白质谱分析提供大量新工具，这将提升我们在疾病诊断方面的能力以及对疾病病理学的理解。