Jiang Xingyu, Bruzewicz Derek A, Wong Amy P, Piel Matthieu, Whitesides George M
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):975-8. doi: 10.1073/pnas.0408954102. Epub 2005 Jan 14.
This report shows that the direction of polarization of attached mammalian cells determines the direction in which they move. Surfaces micropatterned with appropriately functionalized self-assembled monolayers constrain individual cells to asymmetric geometries (for example, a teardrop); these geometries polarize the morphology of the cell. After electrochemical desorption of the self-assembled monolayers removes these constraints and allows the cells to move across the surface, they move toward their blunt ends.
本报告表明,附着的哺乳动物细胞的极化方向决定了它们的移动方向。用适当功能化的自组装单分子层进行微图案化处理的表面将单个细胞限制在不对称的几何形状中(例如泪滴形);这些几何形状使细胞形态极化。在自组装单分子层进行电化学解吸消除这些限制并允许细胞在表面移动后,它们会朝着钝端移动。