Macián M C, Arahal D R, Garay E, Ludwig W, Schleifer K H, Pujalte M J
Departamento de Microbiología y Ecología, Facultad de Biología, Universitat de València, Campus de Burjassot, 46100 València, Spain.
Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, Campus de Burjassot, 46100 València, Spain.
Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):105-110. doi: 10.1099/ijs.0.63275-0.
A Gram-negative, slightly halophilic, strictly aerobic, chemo-organotrophic bacterium was isolated from Mediterranean sea water near Valencia (Spain). 16S rRNA gene sequence comparisons showed that the isolate represented a separate branch within the alpha-3 subclass of the Proteobacteria, now included within the order 'Rhodobacterales'. Jannaschia helgolandensis was the closest relative, but their low sequence similarity and other features indicated that they were not related at the genus level. Isolate 5SM22T produced bacteriochlorophyll a and grew on solid media as regular salmon-pink colonies. Cells are motile rods, with polar flagella. The DNA G+C content is 59.1 mol%. Morphological, physiological and genotypic differences from related, thus far known genera support the description of Thalassobacter stenotrophicus gen. nov., sp. nov. with strain 5SM22T (=CECT 5294T=DSM 16310T) as the type strain.
从西班牙巴伦西亚附近的地中海海水中分离出一株革兰氏阴性、微嗜盐、严格需氧、化能有机营养型细菌。16S rRNA基因序列比较表明,该分离株代表变形菌门α-3亚类中的一个独立分支,现归入“红杆菌目”。赫氏扬氏菌是其最接近的亲缘种,但它们较低的序列相似性和其他特征表明,在属水平上它们并无关联。分离株5SM22T产生细菌叶绿素a,在固体培养基上生长形成规则的鲑鱼粉红色菌落。细胞为具极鞭毛的能动杆菌。DNA的G+C含量为59.1 mol%。与相关的已知属在形态、生理和基因型上的差异支持了窄养海杆菌属(Thalassobacter)的新属描述,新种为嗜窄养海杆菌(Thalassobacter stenotrophicus),模式菌株为5SM22T(=CECT 5294T=DSM 16310T)。