Comparative Genomics of Including the Description of sp. nov., and sp. nov.

A taxogenomic study was conducted to describe two new species and to analyze the internal consistency of the genus along with and . Strains CECT 5113, CECT 5114, CECT 5118, and CECT 5120 were isolated from coastal Mediterranean seawater, Spain. Cells were Gram-negative, non- motile coccobacilli, aerobic chemoorganotrophs, with an optimum temperature of 26°C and salinity of 3.5-5%. Major cellular fatty acids of strains CECT 5113 and CECT 5114 were C ω7c/ω6c and C 3OH, G+C content was 54.4-54.5 mol% and were able to utilize propionate, L-threonine, L- arginine, and L-aspartate as carbon sources. They exhibited 98.3% 16S rRNA gene sequence similarity, 75.0-75.1 ANIb and 19.5-20.9 digital DDH to type strain of their closest species, . Based on these data, strains CECT 5113 and CECT 5114 are recognized as a new species, for which the name is proposed, with strain CECT 5113 (=LMG 29900) as type strain. Strains CECT 5118 and CECT 5120 were found to constitute another new species, with major cellular fatty acids C ω/ω and C ω 11-methyl and a G+C content of 59.8 mol%; they were not able to utilize propionate, L-threonine, L- arginine or L-aspartate. Their closest species was , with values of 99.6% 16S rRNA gene sequence similarity, 79.1% ANIb and 23.2% digital DDH compared to the type strain, CECT 5383. The name is proposed for this second new species, with strain CECT 5118 (=LMG 29904) as type strain. To better determine the phylogenetic relationship of the two new species, we submitted 12 genomes representing species of , and , to a phylogenomic analysis based on 54 single protein-encoding genes (BCG54). The resulting phylogenomic tree did not agree with the current genera classification, as was divided in three clades, sensu stricto ( sp. nov., and ), plus the three spp (, and sp. SK013) and finally, plus sp. nov. remained apart from the two genera. Phenotypic inferences from explored genomes are presented.