从纤细裸藻中纯化芳香多酶聚集体。
Purification of the arom multienzyme aggregate from Euglena gracilis.
作者信息
Patel V B, Giles N H
出版信息
Biochim Biophys Acta. 1979 Mar 16;567(1):24-34. doi: 10.1016/0005-2744(79)90168-2.
PMID:156559
Abstract
The arom multienzyme complex that catalyzes steps two through six in the prechorismate polyaromatic amino acid biosynthetic pathway has been purified up to 2000-fold from Euglena gracilis. The native arom aggregate has a molecular weight of approx. 249 000 based on a sedimentation coefficient of 9.5 and Stokes radius of 60 angstrom. A comparison between the arom aggregates of Neurospora crassa and Euglena gracilis and the possible phylogenetic relationships between the organisms are discussed.
摘要
已从纤细裸藻中纯化出催化分支酸前体多环芳香族氨基酸生物合成途径中第二步至第六步反应的芳香多酶复合物，纯化倍数高达2000倍。根据沉降系数9.5和斯托克斯半径60埃，天然芳香聚集体的分子量约为249000。本文讨论了粗糙脉孢菌和纤细裸藻的芳香聚集体之间的比较以及这两种生物之间可能的系统发育关系。
Zotero 插件