Catledge Shane A, Vohra Yogesh K, Bellis Susan L, Sawyer Amber A
Department of Physics, University of Alabama at Birmingham, Birmingham, Alabama 35242-1170, USA.
J Nanosci Nanotechnol. 2004 Nov;4(8):986-9. doi: 10.1166/jnn.2004.137.
Bone marrow-derived human mesenchymal stem cells were seeded in serum-free media onto ion beam-deposited nanostructured metalloceramic (Ti-Cr-N) films and plasma-nitrided titanium disks, which were left uncoated as well as precoated with fetal bovine serum. Precoating the disks with serum appears to stimulate cell spreading on both the titanium nitride and metalloceramic materials for as little as 1 hour incubation time. The implication is that both of these materials can adsorb serum proteins in amounts sufficient to influence cell adhesion and spreading for potentially improved in vivo response of orthopedic and dental implants. The materials in this study may prove to exhibit enhanced biological and mechanical properties when compared to conventional micron-scale implant materials such as titanium or cobalt-chrome alloys.
将源自骨髓的人间充质干细胞接种于无血清培养基中，接种到离子束沉积的纳米结构金属陶瓷（Ti-Cr-N）薄膜和等离子体氮化钛盘上，这些钛盘未涂层以及预先用胎牛血清包被。用血清预先包被钛盘似乎能刺激细胞在氮化钛和金属陶瓷材料上展开，孵育时间短至1小时即可。这意味着这两种材料都能吸附足够量的血清蛋白，从而影响细胞黏附和展开，有可能改善骨科和牙科植入物的体内反应。与传统的微米级植入材料如钛或钴铬合金相比，本研究中的材料可能表现出增强的生物学和机械性能。
