Forget G, Latxague L, Héroguez V, Labrugère C, Durrieu M C
INSERM U577 laboratory, University of Bordeaux 1, Bordeaux, France.
Annu Int Conf IEEE Eng Med Biol Soc. 2007;2007:5107-10. doi: 10.1109/IEMBS.2007.4353489.
Titanium alloys exhibit excellent biocompatibility and corrosion resistance in the body fluid and possess mechanical properties similar of the bones' properties. When the loss of osseous is important in osseous surgery, large biomaterials are implanted and should be accepted by the organism. For increasing the biomaterials biocompatibility, biological compounds can be linked or deposited on the material surface making them biologically active. In order to study the tissue-implant interaction and to favor osteoblast-adhesion onto titanium, our work deals with the grafting of cell-binding peptides containing the Arginine-Glycine-Aspartic acid (RGD) sequence. In the present study, we focus on the elaboration of patterned biomaterial surfaces with highly functionalized nanodomains. The strategy of RGD peptide immobilization involves first the grafting if an amino-functional organosilane (APTES). Then, each of the free amino moieties were used as an initiator core for a dendrimer-like synthesis to multiply the number of free groups available for RGD immobilization on the material surface.
钛合金在体液中表现出优异的生物相容性和耐腐蚀性，并且具有与骨骼性能相似的机械性能。在骨外科手术中，当骨质流失严重时，需要植入大型生物材料，且这些材料应能被机体接受。为了提高生物材料的生物相容性，可以将生物化合物连接或沉积在材料表面，使其具有生物活性。为了研究组织与植入物之间的相互作用，并促进成骨细胞在钛上的黏附，我们的工作涉及含有精氨酸 - 甘氨酸 - 天冬氨酸（RGD）序列的细胞结合肽的接枝。在本研究中，我们专注于制备具有高度功能化纳米域的图案化生物材料表面。RGD肽固定的策略首先涉及氨基官能化有机硅烷（APTES）的接枝。然后，每个游离氨基部分用作树枝状聚合物样合成的引发剂核心，以增加可用于在材料表面固定RGD的游离基团数量。
