Lai Min, Cai Kaiyong, Hu Yan, Yang Xiaofang, Liu Qing
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Colloids Surf B Biointerfaces. 2012 Sep 1;97:211-20. doi: 10.1016/j.colsurfb.2012.04.029. Epub 2012 Apr 26.
The study describes the influence of surface nanostructured titanium substrates on the growth behaviors of mesenchymal stem cells. Surface nanostructures of titanium were produced with surface mechanical attrition treatment (SMAT) technique. The morphologies of native titanium and surface nanostructured titanium substrates were characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and contact-angle measurements, respectively. A thin nanostructured layer was formed onto the surfaces of titanium substrates after SMAT treatment. The effects of the surface nanostructured titanium substrates on the adhesion, spreading, proliferation and differentiation of mesenchymal stem cells (MSCs) was examined at cellular and molecular levels in vitro. The results suggest that the surface nanostructured substrates were beneficial for the growth of MSCs, including adhesion, filament orientation, proliferation and gene expression. This approach for the fabrication of surface nanostructured titanium may be exploited in the development of high performance titanium-based implants.
该研究描述了表面纳米结构化钛基底对间充质干细胞生长行为的影响。采用表面机械研磨处理（SMAT）技术制备了钛的表面纳米结构。分别用场发射扫描电子显微镜（FE-SEM）、原子力显微镜（AFM）、透射电子显微镜（TEM）、X射线衍射（XRD）和接触角测量对天然钛和表面纳米结构化钛基底的形貌进行了表征。经过SMAT处理后，在钛基底表面形成了一层薄的纳米结构层。在细胞和分子水平上体外研究了表面纳米结构化钛基底对间充质干细胞（MSCs）黏附、铺展、增殖和分化的影响。结果表明，表面纳米结构化基底有利于MSCs的生长，包括黏附、丝状伪足取向、增殖和基因表达。这种制备表面纳米结构化钛的方法可用于高性能钛基植入物的开发。
