Li Xianggan, Nicholl David
Syngenta Biotechnology, Inc, PO Box 12257, 3054 Cornwallis Road, Research Triangle Park, North Carolina 27709-2257, USA.
Pest Manag Sci. 2005 Mar;61(3):277-85. doi: 10.1002/ps.1011.
Recent progress in the development of protoporphyrinogen oxidase (PPO, Protox) inhibitor-resistant plant cell cultures and crops is reviewed, with emphasis on the molecular and cellular aspects of this topic. PPO herbicide-resistant maize plants have been reported, along with the isolation of plant PPO genes and the isolation of herbicide-resistant mutants. At the same time, PPO inhibitor-resistant rice plants have been developed by expression of the Bacillus subtilis PPO gene via targeting the gene into either chloroplast or cytoplasm. Other attempts to develop PPO herbicide-resistant plants include conventional tissue culture methods, expression of modified co-factors of the protoporphyrin IX binding subunit proteins, over-expression of wild-type plant PPO gene, and engineering of P-450 monooxygenases to degrade the PPO inhibitor.
本文综述了抗原卟啉原氧化酶（PPO，原毒素）抑制剂植物细胞培养物和作物开发方面的最新进展，重点关注该主题的分子和细胞层面。已报道了抗PPO除草剂的玉米植株，以及植物PPO基因的分离和抗除草剂突变体的分离。同时，通过将枯草芽孢杆菌PPO基因靶向导入叶绿体或细胞质来培育抗PPO抑制剂的水稻植株。开发抗PPO除草剂植物的其他尝试包括传统组织培养方法、原卟啉IX结合亚基蛋白修饰辅因子的表达、野生型植物PPO基因的过表达以及工程改造P-450单加氧酶以降解PPO抑制剂。
