Sixt Michael, Kanazawa Nobuo, Selg Manuel, Samson Thomas, Roos Gunnel, Reinhardt Dieter P, Pabst Reinhard, Lutz Manfred B, Sorokin Lydia
Department for Experimental Pathology, Lund University, 22185 Lund, Sweden.
Immunity. 2005 Jan;22(1):19-29. doi: 10.1016/j.immuni.2004.11.013.
Resident dendritic cells (DC) within the T cell area of the lymph node take up soluble antigens that enter via the afferent lymphatics before antigen carrying DC arrive from the periphery. The reticular network within the lymph node is a conduit system forming the infrastructure for the fast delivery of soluble substances from the afferent lymph to the lumen of high endothelial venules (HEVs). Using high-resolution light microscopy and 3D reconstruction, we show here that these conduits are unique basement membrane-like structures ensheathed by fibroblastic reticular cells with occasional resident DC embedded within this cell layer. Conduit-associated DC are capable of taking up and processing soluble antigens transported within the conduits, whereas immigrated mature DC occur remote from the reticular fibers. The conduit system is, therefore, not a closed compartment that shuttles substances through the lymph node but represents the morphological equivalent to the filtering function of the lymph node.
淋巴结T细胞区内的驻留树突状细胞（DC）会摄取通过输入淋巴管进入的可溶性抗原，此时携带抗原的DC尚未从外周抵达。淋巴结内的网状网络是一个管道系统，构成了将可溶性物质从输入淋巴管快速输送至高内皮微静脉（HEV）管腔的基础设施。通过高分辨率光学显微镜和三维重建，我们在此表明，这些管道是独特的基底膜样结构，由成纤维网状细胞包裹，偶尔有驻留DC嵌入该细胞层。与管道相关的DC能够摄取和处理在管道内运输的可溶性抗原，而迁移来的成熟DC则远离网状纤维。因此，管道系统不是一个通过淋巴结穿梭物质的封闭隔室，而是在形态上等同于淋巴结的过滤功能。
