在系统神经生物学中实现微阵列技术潜力方面的进展。

Progress in realizing the promise of microarrays in systems neurobiology.

作者信息

Dougherty Joseph D, Geschwind Daniel H

机构信息

Interdepartmental Program in the Neurosciences, University of California at Los Angeles, Los Angeles, CA 90095, USA.

出版信息

Neuron. 2005 Jan 20;45(2):183-5. doi: 10.1016/j.neuron.2005.01.007.

DOI:10.1016/j.neuron.2005.01.007

PMID:15664168

Abstract

The power of microarrays in neuroscience has been challenged by the cellular heterogeneity and complexity of the central nervous system. In this issue of Neuron, Arlotta, Molyneaux, and colleagues have developed a technique combining retrograde labeling, flow cytometry, and microarrays to purify and molecularly characterize a specific population of neurons from the brain, focusing here on cortical projection neurons. We discuss these findings and the implications of this development for both systems and molecular neuroscience.

摘要

微阵列技术在神经科学领域的作用受到了中枢神经系统细胞异质性和复杂性的挑战。在本期《神经元》杂志中，阿洛塔、莫利纽克斯及其同事开发了一种将逆向标记、流式细胞术和微阵列技术相结合的方法，用于从大脑中纯化特定神经元群体并对其进行分子特征分析，这里重点研究皮质投射神经元。我们将讨论这些研究结果以及这一进展对系统神经科学和分子神经科学的意义。