Ciçek Yasin, Ozmen Ismail, Canakçi Varol, Dilsiz Alparslan, Sahin Fikrettin
Department of Periodontology, Ataturk University, Faculty of Dentistry, 25240 Erzurum, Turkey.
Prostaglandins Leukot Essent Fatty Acids. 2005 Mar;72(3):147-51. doi: 10.1016/j.plefa.2004.10.013.
The balance of essential fatty acid is important for good health and normal development. Essential fatty acids (EFA) are the precursors of prostaglandins (PGs), thromboxanes and leukotrienes (LT). The aim of this clinical study was to determine the total fatty acid level of polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), saturated fatty acids (SFA) and each fatty acids level of inflamed and normal gingival tissues. Twenty-seven subjects were included the present study. Nineteen samples of inflamed human gingival tissue (nine of fibrous hyperplasia (FH), ten of peripheral giant cell granuloma (PGCG) and eight samples of normal human gingival tissue were analyzed. The characteristics of inflammation were assessed histologically. Variance analyses were performed to assess the differences among tissues. The total cellular fatty acid profiles of the tissues in inflamed human gingival tissue and in eight samples of normal human gingival tissue were determined by gas chromatography using Sherlock microbial identification system (MIS) software (Microbial ID, Newark, DE, USA) with a database of FAME profiles for eukary. PUFAs, MUFAs, and SFAs were quantified by Sherlock microbial identification system (MIS) or database gas chromatography (DGC). There were statistically significant differences between the concentrations in inflamed (FH, PGCG) and healthy gingival tissues for PUFA and MUFA (P<0.001, P<0,011, respectively). There were statistically significant differences among the concentrations in FH, PGCG, and healthy gingival tissues for SFA (P<0.0001). Arachidonic acid, docosahexaenoic acid, linoleic acid were increased in inflamed tissue. The results of this study showed that unsaturated fatty acids (PUFA and MUFA) significantly increased in inflamed gingival tissues.
必需脂肪酸的平衡对身体健康和正常发育至关重要。必需脂肪酸（EFA）是前列腺素（PGs）、血栓素和白三烯（LT）的前体。本临床研究的目的是测定多不饱和脂肪酸（PUFA）、单不饱和脂肪酸（MUFA）、饱和脂肪酸（SFA）的总脂肪酸水平以及发炎和正常牙龈组织中每种脂肪酸的水平。本研究纳入了27名受试者。分析了19份发炎的人类牙龈组织样本（9份纤维增生（FH）样本、10份外周巨细胞肉芽肿（PGCG）样本）以及8份正常人类牙龈组织样本。通过组织学评估炎症特征。进行方差分析以评估组织间的差异。使用Sherlock微生物鉴定系统（MIS）软件（美国特拉华州纽瓦克市的Microbial ID公司）和真核生物脂肪酸甲酯（FAME）谱数据库，通过气相色谱法测定发炎的人类牙龈组织和8份正常人类牙龈组织样本中组织的总细胞脂肪酸谱。PUFA和MUFA在发炎（FH、PGCG）和健康牙龈组织中的浓度存在统计学显著差异（分别为P<0.001、P<0.011）。SFA在FH、PGCG和健康牙龈组织中的浓度存在统计学显著差异（P<0.0001）。发炎组织中花生四烯酸、二十二碳六烯酸、亚油酸增加。本研究结果表明，不饱和脂肪酸（PUFA和MUFA）在发炎的牙龈组织中显著增加。
