Mesgarzadeh Ali Hossein, Akbarzadeh Abolfazl, Rasipour Ali, Rasipour Tannaz, Mehdizadeh Amir, Shaaker Maghsod
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Cancer Cell Int. 2017 Apr 27;17:50. doi: 10.1186/s12935-017-0414-x. eCollection 2017.
Oral reactive lesions are the most common lesions of oral cavity. Phospholipases and fatty acids play key roles in the creation of inflammation by change in metabolic activities and production of lipid mediators. The aim of this study was to investigate the amount of secretory phospholipase-A2 (sPLA2) and difference of fatty acid pattern in oral reactive hyperplasia and adjacent normal appearing tissues in patients with oral reactive lesions.
Paired samples of oral hyperplastic (OH) and adjacent normal-appearing tissue of 45 patients were investigated in this study. The collected samples were analyzed with enzymatic spectrophotometric method in terms of the amount of sPLA2 and composition of fatty acids by gas-liquid chromatography method.
The amount of sPLA2 (1.8-fold, p < 0.001), stearic acid (1.2-fold, p < 0.001), oleic acid (1.1-fold, p = 0.01), arachidonic acid (1.5-fold, p < 0.001) and docosahexaenoic acid (1.3-fold, p = 0.02) were increased, while the amount of palmitoleic acid (-45%, p < 0.001) and linoleic acid (-19%, p < 0.001) were reduced in the OH tissue samples. Furthermore, the results demonstrated significant associations between the type and location of tissue samples with monounsaturated fatty acids (MUFAs) and n-3 polyunsaturated fatty acids. Tissue samples from patients with inflammatory fibroepithelial hyperplasia showed relatively higher MUFAs and lower n-3 polyunsaturated fatty acids than other type of lesions.
Localized changes in the sPLA2 activity and composition of fatty acid are associated with oral reactive hyperplasia and the type of pathological response. We suggest that sPLA2 activity and multiple type of fatty acids might be used as potential therapeutic target for oral reactive hyperplasia.
口腔反应性病变是口腔最常见的病变。磷脂酶和脂肪酸通过代谢活动的改变和脂质介质的产生在炎症形成中起关键作用。本研究的目的是调查口腔反应性病变患者口腔反应性增生组织及相邻外观正常组织中分泌型磷脂酶A2（sPLA2）的含量及脂肪酸模式的差异。
本研究对45例患者的口腔增生（OH）组织及相邻外观正常组织的配对样本进行了研究。采用酶分光光度法分析所收集样本中sPLA2的含量，并采用气液色谱法分析脂肪酸的组成。
OH组织样本中sPLA2的含量（增加1.8倍，p<0.001）、硬脂酸（增加1.2倍，p<0.001）、油酸（增加1.1倍，p=0.01）、花生四烯酸（增加1.5倍，p<0.001）和二十二碳六烯酸（增加1.3倍，p=0.02）均升高，而棕榈油酸（降低45%，p<0.001）和亚油酸（降低19%，p<0.001）的含量降低。此外，结果表明组织样本的类型和位置与单不饱和脂肪酸（MUFAs）和n-3多不饱和脂肪酸之间存在显著关联。与其他类型的病变相比，炎症性纤维上皮增生患者的组织样本显示出相对较高的MUFAs和较低的n-3多不饱和脂肪酸。
sPLA2活性和脂肪酸组成中的局部变化与口腔反应性增生及病理反应类型有关。我们认为，sPLA2活性和多种脂肪酸可能作为口腔反应性增生的潜在治疗靶点。
