Soydan A S, Dokmetas H S, Cetin M, Koyuncu A, Kaptanoglu E, Elden H
Department of Pharmacology, School of Medicine, Cumhuriyet University, Sivas, Turkey.
Mediators Inflamm. 2006;2006(5):64980. doi: 10.1155/MI/2006/64980.
Beta-hydroxy fatty acids are a major component of lipid A moiety of lipopolysaccharide. We aimed to investigate the role of free beta-hydroxy fatty acids on inflammation, as well as to evaluate their effects on cytokine release from human blood cells, and whether they exist in plasma of patients with chronic inflammatory diseases with/without insulin resistance. Peripheral venous blood was incubated with beta-hydroxy lauric and beta-hydroxy myristic acids (each 100 ng, 1 microg, 10 microg/mL) up to 24 hours. Cytokines were measured from culture media and plasma. Free fatty acids and biochemical parameters were also measured from patients' plasma. Only beta-hydroxy lauric acid significantly stimulated interleukin-6 production at 10 microg/mL compared to control (533.9 +/- 218.1 versus 438.3 +/- 219.6 pg/mL, P < .05). However, free beta-hydroxy lauric and myristic acids were not found in patients' plasma. Therefore, free beta-hydroxy lauric and myristic acids do not seem to have a role on sterile inflammation in chronic inflammatory diseases associated with insulin resistance.
β-羟基脂肪酸是脂多糖脂质A部分的主要成分。我们旨在研究游离β-羟基脂肪酸在炎症中的作用，评估它们对人血细胞释放细胞因子的影响，以及它们是否存在于伴有或不伴有胰岛素抵抗的慢性炎症性疾病患者的血浆中。将外周静脉血与β-羟基月桂酸和β-羟基肉豆蔻酸（各100 ng、1 μg、10 μg/mL）孵育长达24小时。从培养基和血浆中测量细胞因子。还从患者血浆中测量游离脂肪酸和生化参数。与对照组相比，仅β-羟基月桂酸在10 μg/mL时显著刺激白细胞介素-6的产生（533.9±218.1对438.3±219.6 pg/mL，P<.05）。然而，在患者血浆中未发现游离β-羟基月桂酸和肉豆蔻酸。因此，游离β-羟基月桂酸和肉豆蔻酸似乎在与胰岛素抵抗相关的慢性炎症性疾病的无菌性炎症中不起作用。
