阿拉伯白花菜叶提取物、芦丁和槲皮素对大豆脂氧合酶活性及人中性粒细胞炎性类二十烷酸生成的影响。
Effect of Cleome arabica leaf extract, rutin and quercetin on soybean lipoxygenase activity and on generation of inflammatory eicosanoids by human neutrophils.
作者信息
Bouriche H, Miles E A, Selloum L, Calder P C
机构信息
Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences, University of Ferhat Abbas, 19000 Setif, Algeria.
出版信息
Prostaglandins Leukot Essent Fatty Acids. 2005 Mar;72(3):195-201. doi: 10.1016/j.plefa.2004.10.018.
The effects of Cleome arabica leaf extract, rutin and quercetin on soybean lipoxygenase (Lox) activity and on calcium ionophore (A23187)-stimulated generation of the leukotriene B4 and prostaglandin E2 by human neutrophils were examined. The extract (25 microg/ml), rutin (25 microM) and quercetin (25 microM) inhibited LTB4 synthesis at all concentrations of A23187 used. The extract at 1-100 microg/ml and rutin at 1-100 microM inhibited LTB4 generation by neutrophils stimulated with 1 microM A23187 by about 50%. PGE2 production in response to different concentrations of A23187 was affected in a biphasic manner by the extract and rutin. Quercetin at 1-100 microM caused concentration-dependent inhibition of LTB4 and PGE2 production. The extract, rutin and quercetin caused concentration-dependent inhibition of soybean Lox activity. These results indicate that rutin, quercetin and an extract of C. arabica containing these compounds inhibit Lox activity, consequently decreasing LTB4 production. Thus, these compounds or extracts containing them may be beneficial for the treatment of inflammatory conditions, particularly those characterised by excessive leukotriene generation.
研究了阿拉伯白花菜叶提取物、芦丁和槲皮素对大豆脂氧合酶（Lox）活性以及对钙离子载体（A23187）刺激人中性粒细胞生成白三烯B4和前列腺素E2的影响。提取物（25微克/毫升）、芦丁（25微摩尔）和槲皮素（25微摩尔）在所用的所有A23187浓度下均抑制白三烯B4的合成。1至100微克/毫升的提取物和1至100微摩尔的芦丁抑制1微摩尔A23187刺激的中性粒细胞生成白三烯B4约50%。提取物和芦丁以双相方式影响对不同浓度A23187的前列腺素E2产生。1至100微摩尔的槲皮素引起白三烯B4和前列腺素E2产生的浓度依赖性抑制。提取物、芦丁和槲皮素引起大豆Lox活性的浓度依赖性抑制。这些结果表明，芦丁、槲皮素以及含有这些化合物的阿拉伯白花菜提取物抑制Lox活性，从而减少白三烯B4的产生。因此，这些化合物或含有它们的提取物可能有益于炎症性疾病的治疗，尤其是那些以白三烯过度产生为特征的疾病。
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