Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2d, 15-222 Bialystok, Poland.
Nutrients. 2020 Apr 13;12(4):1074. doi: 10.3390/nu12041074.
The combination of ascorbic acid and rutin, commonly used in oral preparations for their antioxidant and anti-inflammatory properties, can also be used to protect skin cells from the effects of UV radiation in sunlight. Here, we tested the potential protective effect of ascorbic acid and rutin used together in UVB-irradiated human skin fibroblasts, and assessed the proteomic profile of these cells, grown in a three-dimensional (3D) system. Proteomic findings revealed a combined effect of ascorbic acid and rutin in UV-irradiated fibroblasts against overexpression of pro-inflammatory signaling proteins and DNA reorganization/expression. These effects were not observed when cells were treated with either compounds alone. The antioxidant effects of ascorbic acid and rutin also prevented protein modifications by lipid peroxidation products. Further, ascorbic acid stimulated rutin-protein adduct formation, which supports intra/extracellular signaling and the Nrf2/ARE antioxidant pathway, contributing to the protective effects against UV-induced oxidative stress. The combined effect of ascorbic acid and rutin suggests that this combination of compounds is potentially effective against skin damage caused by UV radiation.
抗坏血酸和芦丁的组合，通常因其抗氧化和抗炎特性而被用于口服制剂中，也可以用于保护皮肤细胞免受阳光中 UV 辐射的影响。在这里，我们测试了抗坏血酸和芦丁在 UVB 照射的人皮肤成纤维细胞中的联合保护作用，并评估了这些细胞在三维（3D）系统中的蛋白质组学特征。蛋白质组学研究结果表明，抗坏血酸和芦丁联合使用对 UV 照射的成纤维细胞中促炎信号蛋白的过度表达和 DNA 重组/表达具有联合作用。当细胞单独用任一化合物处理时，未观察到这些作用。抗坏血酸的抗氧化作用还防止了脂质过氧化产物引起的蛋白质修饰。此外，抗坏血酸刺激芦丁-蛋白质加合物的形成，这支持细胞内/细胞外信号和 Nrf2/ARE 抗氧化途径，有助于对抗 UV 诱导的氧化应激的保护作用。抗坏血酸和芦丁的联合作用表明，这种化合物的组合可能对由 UV 辐射引起的皮肤损伤有效。
