Venkatesh Palani, Daggumati Mahesh
Mother Theresa Post Graduate and Research Institute of Health Sciences, Pondicherry University, Pondicherry 605006, India.
KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore 641048, India.
J Pharm Anal. 2012 Apr;2(2):152-155. doi: 10.1016/j.jpha.2011.11.002. Epub 2011 Nov 10.
Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated. The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2, v/v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 276 nm. The values were 0.41±0.03 and 0.60±0.04 for Lamivudine and Zidovudine, respectively. The linearity of the method was found to be within the concentration range of 50-250 ng/spot for Lamivudine and for Zidovudine, it was 100-500 ng/spot. The lower limits of detection and quantification were 2.23 ng/spot and 7.90 ng/spot for Lamivudine and 2.90 ng/spot and 8.85 ng/spot for Zidovudine. The method was also validated for precision, specificity and recovery. This developed method was used to analyze fixed-dose tablets (Duovir, Cipla Ltd) samples of Lamivudine and Zidovudine.
采用高效薄层色谱法（HPTLC）同时测定片剂中拉米夫定和齐多夫定的含量，并进行了方法验证。在硅胶GF铝预涂薄层板上，以甲苯∶乙酸乙酯∶甲醇（4∶4∶2，v/v/v）为流动相展开色谱图，于276 nm波长处采用密度计吸光度模式进行定量分析。拉米夫定和齐多夫定的比移值（Rf）分别为0.41±0.03和0.60±0.04。该方法的线性范围为：拉米夫定50 - 250 ng/点，齐多夫定100 - 500 ng/点。拉米夫定的检测限和定量下限分别为2.23 ng/点和7.90 ng/点，齐多夫定的检测限和定量下限分别为2.90 ng/点和8.85 ng/点。对该方法的精密度、特异性和回收率也进行了验证。所建立的方法用于分析拉米夫定和齐多夫定的复方制剂（双汰芝，西普拉有限公司）样品。
