Le Guillou-Buffello Delphine, Hélary Gérard, Gindre Marcel, Pavon-Djavid Graciela, Laugier Pascal, Migonney Véronique
Laboratoire d'Imagerie Paramétrique, UMR 7623 CNRS, Université Pierre et Marie Curie, 15 rue de l'école de Médecine, 75006 Paris, France.
Biomaterials. 2005 Jul;26(19):4197-205. doi: 10.1016/j.biomaterials.2004.10.024.
The Thickness Shear Mode (TSM) quartz crystal resonator has been extensively used as sensitive sensor in various electrochemical and biological applications. This technique based on the propagation of an ultrasonic shear wave generated by a sinusoidal electric field through a piezoelectric quartz resonator, provides a non-destructive and powerful means to probe changes at solid-solid or solid-liquid interfaces. In this study, TSM was used to characterize cell-polymer interactions developing during the cell adhesion process. TSM sensing was used to monitor the inhibiting properties of bioactive polymers towards fibroblast McCoy adhesion processes. For this purpose, thin films of various bioactive polymers exhibiting either carboxylate or/and sulfonate functional groups were deposited onto the TSM. Measurements of the time variation of the electrical motional resistance in the vicinity of the mechanical sensor resonant frequency were performed as the quartz crystal resonator was either coated with the continuous polymer phase or polymer plus cell suspensions. Cell adhesion processes on these surfaces was investigated by cell counting and the quartz resonator-based technique. Inhibition of fibroblast McCoy adhesion onto thin polymer films of various chemical compositions was analyzed and discussed in the perspective of a possible application of these bioactive polymers to fabricate intraocular lenses able to prevent secondary cataract phenomena.
厚度剪切模式（TSM）石英晶体谐振器已被广泛用作各种电化学和生物应用中的敏感传感器。该技术基于正弦电场通过压电石英谐振器产生的超声剪切波的传播，提供了一种无损且强大的手段来探测固-固或固-液界面处的变化。在本研究中，TSM用于表征细胞粘附过程中发生的细胞-聚合物相互作用。TSM传感用于监测生物活性聚合物对成纤维细胞 McCoy 粘附过程的抑制特性。为此，将具有羧酸盐或/和磺酸盐官能团的各种生物活性聚合物薄膜沉积在TSM上。当石英晶体谐振器涂覆有连续聚合物相或聚合物加细胞悬浮液时，在机械传感器谐振频率附近测量电运动电阻的时间变化。通过细胞计数和基于石英谐振器的技术研究了这些表面上的细胞粘附过程。从这些生物活性聚合物在制造能够预防继发性白内障现象的人工晶状体中的可能应用的角度，分析和讨论了成纤维细胞 McCoy 对各种化学成分的聚合物薄膜的粘附抑制作用。
