用于生物传感器应用的聚合物涂层表面上神经细胞的培养。

Culture of neural cells on polymers coated surfaces for biosensor applications.

作者信息

Lakard S, Herlem G, Valles-Villareal N, Michel G, Propper A, Gharbi T, Fahys B

机构信息

Laboratory of Chemistry, Materials and Interfaces, University of Franche-Comté, 16 route de Gray, 25030 Besançon, France.

出版信息

Biosens Bioelectron. 2005 Apr 15;20(10):1946-54. doi: 10.1016/j.bios.2004.09.001.

DOI:10.1016/j.bios.2004.09.001

PMID:15741062

Abstract

We focused our study on the olfactory cells growth on biocompatible polymer films electrodeposited on a silicon microsystem. Several substrates such as polyethyleneimine (PEI), polypropyleneimine (PPI), and polypyrrole (PPy), acting as potentially good candidates for cell culture, were tested in order to allow cells to adhere and proliferate. During their growth, the evolution of their morphology was monitored using both confocal microscope and immunohistochemistry, leading to the conclusion of a normal development. An estimation of the adhesion and proliferation rates of rat neuronal cell cultures indicated that PEI and PPI were the best substrates for cultivating olfactory cells.

摘要

我们的研究聚焦于在硅微系统上电沉积的生物相容性聚合物薄膜上嗅觉细胞的生长情况。测试了几种底物，如聚乙烯亚胺（PEI）、聚丙烯亚胺（PPI）和聚吡咯（PPy），它们作为细胞培养的潜在良好候选物，以促使细胞黏附和增殖。在细胞生长过程中，使用共聚焦显微镜和免疫组织化学监测其形态演变，得出细胞发育正常的结论。对大鼠神经元细胞培养物的黏附和增殖率的评估表明，PEI和PPI是培养嗅觉细胞的最佳底物。